HPLC-MS/MS法测定人体血浆中奥沙普秦的药物浓度  

Determination of Oxaprozin in Human Plasma by HPLC-MS/MS

作　　者：俞岚[1] 谢云[2] 

机构地区：[1]湖北第二师范学院,湖北武汉430205 [2]湖北中医药大学,湖北武汉430065

出　　处：《今日药学》2016年第4期239-241,264,共4页Pharmacy Today

摘　　要：目的建立人体血浆中奥沙普秦浓度的HPLC-MS/MS分析方法。方法以布洛芬为内标,采用乙腈-20 mmol/L乙酸铵缓冲溶液（60∶40）为流动相,以AQ-C18（5μm,2.1 mm×100 mm,美国Welch公司）为色谱柱,以电喷雾负离子多反应监测模式检测。结果奥沙普秦血浆浓度在0.5~80μg/m L范围内呈现良好线性关系（r=0.998 4）,定量下限为0.5μg/m L,日内和日间精密度均小于10%,方法学回收率为92.0%~103.55%。结论该方法专属性强、准确、灵敏、快捷,适用于奥沙普秦的临床药动学研究。OBJECTIVE To establish an analytical method for determination of Oxaprozin in human plasma by HPLC-MS / MS.METHODS Ibuprofen was used as internal standard. Oxaprozin was separated on a AQ-C18column（ 5 μm,2.1 mm×100 mm,Welch Material Inc）. The mobile phase consisted of acetonitrile ： ammonium acetate buffer solution（ 60 ∶40）. Quantification was performed by multiple reaction monitoring（ MRM） and electrospray ionization in negative mode. RESULTS Oxaprozin showed a good linearity in the range of 0.5-80 μg / mL（ r = 0.998 4）. The quantitative lower limit was 0.5 μg / mL. The inter-day and intra-day precisions were less than 10%. The method recovery rate was 92. 0%- 103. 55%. CONCLUSION The method is proved to be selective,accurate,sensitive,simple and suitable for pharmacokinetic study of Oxaprozin.

关 键 词：奥沙普秦 血药浓度 高效液相串联质谱方法 

分 类 号：R927.2[医药卫生—药学]