白术利尿作用研究  

Study on Diuretic Effect of Atractylodis Macrocephalae Rhizoma

作　　者：陈静[1] 孙云超[1] 冉小库[1] 袁颖[1] 窦德强[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国现代中药》2016年第5期563-567,共5页Modern Chinese Medicine

基　　金：国家重点基础研究计划(973计划)(2013CB531803);2013辽宁省高等学校创新团队课题(LT2013020)

摘　　要：目的:研究白术及其拆分组分对大鼠的利尿作用。方法:采用多模式联用的方法对白术化学组分进行拆分;通过预先胃水负荷模型,以6 h尿量为指标考察白术及其拆分组分对水负荷大鼠利尿作用;进一步测定红细胞和肾髓中Na^+-K^+-ATP酶活力、血尿素氮浓度、肾髓中碳酸酐酶水平和Na^+、K^+、Cl-排出量,研究其相关机理。结果:高剂量白术水煎液和白术挥发油组分对大鼠有一定的抗利尿作用;与空白组相比,白术水煎液及其拆分组分组红细胞中Na^+-K^+-ATP酶活力、肾髓中Na^+-K^+-ATP酶和碳酸酐酶水平无显著变化。结论:白术对正常动物无利尿作用,相反表现出一定的抗利尿作用,且首次研究发现白术挥发油有一定的抗利尿作用。Objective： To study the diuretic effect of the Atractylodis macrocephalae rhizoma and its fractions. Methods： Muhi-mode separation methods were adopted to split the components of AtractylQdis macrocephalae rhizoma. The indictor of urine excretion in 6 h was used to study the diuretic effect of Atractylodis macrocephalae rhizoma and its splitted fractions in the water loaded rats. Further, the activity of Na^＋ -K ^＋ -ATPase in red cell and renal medulla, the level of carbonic anhydrase in renal medulla and the output of Na^＋, K ^＋ , Cl^- in urinewere measured to elucidate the related mechanization. Results： The anti-diuretic effect was observed in the high dosage of Atractylodis macrocephalae rhizoma water decoction and volatile oil fraction groups. Versus the control group, the activity of Na^＋ -K ^＋ -ATPase in red cell and renal medulla and the level of carbonic anhydrase in renal medulla were not significantly changed in Atractylodis macrocephalae rhizoma water decoction and its fractions groups. Conclusion： The diuretic effect of Atraetylodis macrocephalae rhizoma was not observed, instead, the weak antidiuretic effect was presented and it is for the first time that antidiuretic effect of Atractylodis macroeephalae rhizoma volatile oilwas found.

关 键 词：白术 拆分组分 利尿作用 

分 类 号：R285.5[医药卫生—中药学]