小球藻生长及关键营养元素代谢规律的研究  

Study on the Growth and Metabolisms of the Key Elements for the Cultivation of Chlorella vulgaris

作　　者：纪旭艳 李荣贵[1] 郭欣[2] 黄青山[2] 

机构地区：[1]青岛大学,山东青岛266000 [2]中国科学院青岛生物能源与过程研究所,山东青岛266101

出　　处：《安徽农业科学》2016年第9期1-4,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(21106169;21376254;91434114);国家重点基础研究发展计划项目(2012CB224806)

摘　　要：[目的]探索小球藻生长过程中关键营养元素代谢的基本规律,优化小球藻大规模培养工艺。[方法]以新型、高效的平板气升环流式光生物反应器为模式反应器培养小球藻,定时检测均匀鼓泡流下小球藻的生长状况和培养液中氮、磷、钾、钙、镁、铁等关键营养元素的浓度。[结果]小球藻在264 h内的干重浓度接近线性增加趋势;铁元素在48 h后几乎消耗殆尽;氮和磷元素在168 h后降至较低水平;镁和钙元素在96 h降至较低水平后不再明显下降;钾元素虽然是小球藻生长的重要组成元素,但其浓度始终处于波动状态,变化并不显著。[结论]小球藻的元素代谢主要发生在168 h前,各种元素的代谢速率有显著差异;生长后期虽然大多数营养元素比较缺乏,但在持续光照和通入足够二氧化碳的条件下小球藻仍然可以继续生长一段时间。[Objective] The aim was to explore the basic law of key elements metabolism in the growth process of Chlorella vulgaris,and optimize the large-sclae cultivation process. [Method] A new and high-efficient rectangular airlift loop photobioreactor was employed to produce Chlorella vulgaris,and the concentration curves of the key elements in the culture including N,P,K,Ca,Mg and Fe were measured every day during the cultivation to investigate the metabolic laws under the bubbly flow condition. [Result]The results indicated that the concentration of biomass based on the dry weight increases nearly linearly. The concentration of iron was depleted within 48 hours; Nitrogen and phosphorus dropped to a very low concentration after 168 hours. The concentration of magnesium and calcium were no longer declining after 96 hours,while the concentration of potassium kept unchanged. Nitrogen,phosphorus,magnesium,calcium and iron were essential elements for the growth of Chlorella vulgaris,but potassium was not necessary to be kept with a high concentration. [Conclusion]Most of the key elements are depleted in 168 h,and different element consumption rates are obtained. Although the lack of nutrients at last,the Chlorella vulgaris keeps growing by adding gas with sufficient CO2 and keeping illumination continuously in a limited period.

关 键 词：二氧化碳 小球藻 元素代谢 平板气升环流式光生物反应器 均匀鼓泡流 

分 类 号：S968.4[农业科学—水产养殖]