消化乳杆菌泡菜分离株W369的鉴定及其益生功能  

Identification of a new probiotic Lactobacillus alimentarius W369 from Chinese traditional pickles

作　　者：汤伟[1] 胡维[1] 王菁蕊[1] 王金菊[1] 王艳萍[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,食品营养与安全教育部重点实验室,天津300457

出　　处：《微生物学报》2016年第6期932-942,共11页Acta Microbiologica Sinica

基　　金：国家“863计划”(2011AA100904)~~

摘　　要：【目的】鉴定从中国传统发酵泡菜分离的1株乳酸菌,并分析其潜在的益生功能。【方法】通过形态观察、生理生化、16S rRNA基因序列分析等方法对分离得到的乳酸菌进行鉴定;评价该菌株的NaCl耐受能力、抗生素最小抑制浓度,以及益生功能。【结果】该菌株鉴定为消化乳杆菌(Lactobacillus alimentarius)W369,在10%的NaCl浓度下仍可以存活;对常用抗生素具有不同程度的敏感性。益生功能分析表明,培养72 h后W369对亚硝酸钠的降解率为92.92%;其体外胆固醇脱除率达到31.80%;发酵液上清具有清除DPPH自由基(DPPH·)、超氧阴离子自由基(·O_2~–)和螯合亚铁离子(Fe^(2+))的作用,分别达到(88.02±1.48)%、(43.75±3.10)%和(29.99±2.34)%;完整细胞具有很强的还原能力[(244.52±0.92)μmol/m L半胱氨酸当量]、羟自由基(·OH)清除能力(77.17±0.58)%和抗脂质过氧化的能力(29.67±0.77)%;发酵上清液和无细胞提取物均具有谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(Mn-SOD)活性。【结论】消化乳杆菌W369分离株具有亚硝酸盐降解能力、胆固醇清除能力和抗氧化活性等诸多益生功能,具有潜在的应用价值。[Objective] A new lactic acid bacterium was isolated from Chinese traditional pickles and its potential probiotic properties were analyzed. [Methods] The strain was identified by morphological observation, physiological and biochemical tests and 16 S rRNA gene comparison. Its probiotic properties were evaluated, including resistance to NaCl, the minimum inhibitory concentration of antibiotics, nitrite degradation ability and cholesterol removal ability.[Results] This strain was identified as Lactobacillus alimentarius W369（No. CGMCC 7.180）. L. alimentarius W369 could survive under the concentration of 10% NaCl and it was sensitive to some common antibiotics. By studying its probiotic properties, we found that its nitrite degradation rate reached 92.92% after 72 h and the cholesterol removal rate reached 31.80%. The fermented supernatant showed strong abilities of DPPH· and the superoxide anion radical scavenging capacity, as well as Fe^（2＋） chelating ability. The clearance rate were（88.02±1.48）%,（43.75±3.10）% and（29.99±2.34）% respectively. Strain W369 also exhibited strong reducing capacity, hydroxyl radical scavenging action and lipid peroxidation inhibition capacity. Both the fermented supernatant and cell-free extracts exhibited glutathione peroxidase（GSH-Px） and superoxide dismutase（Mn-SOD） activities. [Conclusion] L. alimentarius W369 possessed many probiotic properties including nitrite degradation ability, cholesterol removal ability and antioxidant activity. It has many potential application values.

关 键 词：消化乳杆菌 降解亚硝酸盐 降胆固醇 抗氧化活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]