2014年甘肃省部分地区蚊虫及蚊媒病毒调查  

Investigation of mosquitoes and mosquito-borne arboviruses in Gansu Province,China

作　　者：于德山[1] 崔世恒 付士红[3] 曹蕾[3] 曹玉玺[3] 陈生邦[1] 李国太[1] 蒋建祥[1] 梁国栋[3] 

机构地区：[1]甘肃省疾病预防控制中心,甘肃730000 [2]青岛大学,青岛266071 [3]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《微生物与感染》2016年第3期153-158,共6页Journal of Microbes and Infections

基　　金：国家自然科学基金(81290342)

摘　　要：为调查甘肃省部分地区蚊虫及蚊媒病毒的种类及分布,本课题组2014年在甘肃省酒泉市和陇南市地区共采集蚊虫4 412只。酒泉市共采集蚊虫2属3种2 833只,其中背点伊蚊(Aedes dorsalis)2 665只(94.1%)、刺扰伊蚊(Aedes vexans)150只(5.3%)、淡色库蚊(Culex pipiens pallens)18只(0.6%);陇南市共采集2属2种1 579只蚊虫,其中淡色库蚊1 451只(91.9%)、中华按蚊(Anopheles sinensis)128只(8.1%)。经实验室常规处理后,将蚊虫标本分为96批,接种于C6/36、BHK和Vero细胞,进行病毒分离和多种病毒基因特异性扩增检测。结果显示,96批蚊虫标本在3种细胞中均未出现细胞病变效应(cytopathic effect,CPE),在18批蚊虫标本研磨液中检测到辽宁病毒(Liaoning virus,LNV)基因阳性。病毒核苷酸序列的遗传进化分析显示,在甘肃省检测到的辽宁病毒与此前在我国新疆维吾尔自治区发现的辽宁病毒亲缘关系最近。The purpose of the current study is to investigate the distribution of mosquitoes and mosquito-borne arboviruses in Gansu Province.The mosquitoes were collected from Jiuquan and Longnan in 2014.2 833 mosquitoes were collected from Jiuquan,including Aedes dorsalis 2 665（94.1%）,Aedes vexans 150（5.3%）and Culex pipiens pallens 18（0.6%）.1 579 mosquitoes were collected from Longnan,including Culex pipiens pallens 1 451（91.9%）and Anopheles sinensis 128（8.1%）.All mosquitoes were divided into 96 pools and then inoculated onto C6/36,BHK and Vero cells for virus isolation and identification.The results showed that the 96 pools of mosquitoes inoculated on three cells revealed negative cytopathic effect（CPE）.Eighteen pools of the mosquito samples showed positive for Liaoning virus（LNV）detection.The genetic evolution analysis of viral nucleotide sequences showed that Liaoning virus in Gansu Province had the closest relationship with the Liaoning virus isolated in Xinjiang Uygur Autonomous Region.

关 键 词：甘肃省 酒泉市 陇南市 蚊虫 蚊媒病毒 辽宁病毒 

分 类 号：R384.1[医药卫生—医学寄生虫学]