Th17细胞及相关因子IL-17与绝经后骨质疏松的相关性研究  

The effect of Th17 and IL-17 on the pathogenesis of postmenopausal osteoporosis

作　　者：刘连勇[1,2] 陆志仁 游利[4] 胡晓晖[5] 

机构地区：[1]上海市浦东新区浦南医院内分泌科,200125 [2]江苏省苏州大学医学院研究生院,215123 [3]上海市宝山区淞南社区服务中心,上海200441 [4]上海市第一人民医院,上海200080 [5]上海市浦东新区浦南医院脊柱外科,200125

出　　处：《中国骨质疏松杂志》2016年第5期545-549,共5页Chinese Journal of Osteoporosis

摘　　要：目的观察辅助性T细胞17（T helper cell 17,Th17）及及相关因子白细胞介素17（interleukin 17,IL17）在绝经后骨质疏松（Postmenopausal osteoporosis,PMOP）患者外周血及血清中的表达水平,并探讨IL-17细胞与绝经后骨质疏松的发病的关系。方法选取2011年7月~2014年7月在我院确诊的绝经后骨质疏松患者（60例）为研究组,选取同期来我院体检的绝经后骨量正常人群为对照组（60例）,采用流式细胞术检测绝经后骨质疏松患者及骨质正常人外周血中Th17细胞水平,采用ELISA法检测两组人群外周血中IL-17、IL-6及TGF-β的蛋白水平;采用RT-PCR法检测两组人群外周血中转录因子T孤独核受体（retinoid-related orphan receptor gamma t,RORγT）的mRNA水平。通过Pearson相关分析绝经后骨质疏松患者中IL-17水平与骨密度（bone mineral density,BMD）以及血清钙可能存在的关系。结果绝经后骨质疏松患者外周血中Th17细胞比例为（2.17±0.41）%,显著高于健康对照组人群的（0.61±0.45）%,差异有统计学意义（P〈0.001）;绝经后骨质疏松患者血清IL-17水平（31.51±10.09）pg/ml,显著高于对照组人群的（16.61±9.93）pg/ml,差异有统计学意义（P〈0.001）;绝经后骨质疏松患者外周血RORγT mRNA的表达水平显著高于正常人群,差异有统计学意义（P〈0.001）。绝经后骨质正常对照组人群外周血血清中IL-6及TGF-β水平均显著低于绝经后骨质疏松患者,差异有统计学意义（t=8.99,P=0.000;t=4.71,P=0.000）;此外,Pearson相关分析结果显示,绝经后骨质疏松患者血清中IL-17与BMD呈显著负相关（r=0.788,P〈0.05）。结论Th17细胞可能通过分泌IL-17促进绝经后骨质疏松的产生,IL-17可能作为治疗绝经后骨质疏松的重要靶点。Objective To observe T helper cell 17（ Th17） and serum interleukin 17（ IL-17） expression in peripheral blood in patients with postmenopausal osteoporosis（ PMOP）,and to explore the relationship between IL-17 cells and the pathogenesis of PMOP. Methods Sixty PMOP patients in our hospital from July 2011 to July 2014 were selected as the study group,and 60 normal postmenopausal people at the same time were selected as the control group. The peripheral blood Th17 cells were detected using flow cytometry. Serum levels of IL 17,IL-6,and TGF-β were detected using ELISA. The mRNA level of retinoid-related orphan receptor gamma T（ RORγT） was detected using RT-PCR in two groups. The relationship between IL-17 and bone mineral density（ BMD）,serum calcium was studied in PMOP. Results Th17 cells in the peripheral blood in PMOP patients（ 2. 17 ±0. 41%） were significantly higher than those in normal people（ 0. 61 ± 0. 45%,P〈0. 001）. IL-17 level in PMOP patients（ 31. 51± 10. 09 pg /ml） was significantly higher than those in normal people（ 16. 61 ± 9. 93 pg /ml,P〈0. 001）. The expression of RORγT mRNA in PMOP patients was significantly higher than that the control group（ P〈0. 001）. In addition,IL-6 and TGF-β in PMOP patients were significantly higher than those in the control group（ P〈0. 001）. Furthermore,Pearson correlation analysis showed that IL-17 was negatively correlated with BMD（ r = 0. 788,P〈0. 05） in PMOP patients. Conclusion Th17 cells promote the pathogenesis of PMOP by secreting IL-7. IL-17 may act as an important target for the treatment of PMOP.

关 键 词：辅助性T细胞17 白细胞介素17 T孤独核受体 雌激素 绝经后骨质疏松 

分 类 号：R68[医药卫生—骨科学]