Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu^2＋ exposure  

作　　者：Xiao-Juan Hu Zhi-Xiao Liu Ya-Di Wang Xue-Ling Li Jun Hu Jun-Hong Lv 

机构地区：[1] Division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology,Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China [2] University of Chinese Academy of Sciences,Beijing 100049,China [3] Shanghai Center for Bioinformation Technology,Shanghai Academy of Science and Technology,Shanghai 201203,China [4] Center for Clinical and Translational Medicine,Shanghai Industrial Technology Institute,Shanghai 201203,China

出　　处：《Nuclear Science and Techniques》2016年第3期45-52,共8页核技术（英文）

基　　金：Supported by National Natural Science Foundation of China(No.11474298);Shanghai Pujiang Program(No.13PJ1410500);Special Funds for Enterprise Independent Innovation of Shanghai(CXY-2013-58);Hundred Talents Program of the Chinese Academy Sciences

摘　　要：Copper ions(e.g.,Cu^(2+)) have outstanding antibacterial properties,but the exact mechanism is rather complex and not fully understood.In this work,synchrotron Fourier transform infrared(FTIR) spectroscopy was used as an analytical tool to investigate the CuCl_2-induced biochemical changes in Escherichia coli.Our spectral measurements indicated that this technique is sensitive enough to detect changes in membrane lipids,nucleic acids,peptidoglycans and proteins of Cu^(2+)-treated bacteria.Interestingly,for short-time treated cells,the effects on phospholipid composition were clearly shown,while no significant alterations of proteins,nucleic acids and peptidoglycans were found.PeakForce quantitative nano-mechanics mode atomic force microscopy(AFM)confirmed the changes in the topography and mechanical properties of bacteria upon the Cu^(2+) exposure.This study demonstrated that FTIR spectroscopy combined with AFM can provide more comprehensive evaluation on the biochemical and mechanical responses of bacteria to copper.Copper ions（e.g.,Cu^2＋） have outstanding antibacterial properties,but the exact mechanism is rather complex and not fully understood.In this work,synchrotron Fourier transform infrared（FTIR） spectroscopy was used as an analytical tool to investigate the CuCl2-induced biochemical changes in Escherichia coli.Our spectral measurements indicated that this technique is sensitive enough to detect changes in membrane lipids,nucleic acids,peptidoglycans and proteins of Cu^2＋-treated bacteria.Interestingly,for short-time treated cells,the effects on phospholipid composition were clearly shown,while no significant alterations of proteins,nucleic acids and peptidoglycans were found.PeakForce quantitative nano-mechanics mode atomic force microscopy（AFM）confirmed the changes in the topography and mechanical properties of bacteria upon the Cu^2＋ exposure.This study demonstrated that FTIR spectroscopy combined with AFM can provide more comprehensive evaluation on the biochemical and mechanical responses of bacteria to copper.

关 键 词：CUCL2 红外光谱 大肠杆菌 同步 原子力显微镜 FTIR光谱 分子 曝光 

分 类 号：Q93-33[生物学—微生物学]