怀地黄中地黄苷D的提取工艺及不同炮制品含量的比较研究  

Study on Extraction Process of Rehmannioside D in Radix Rehmanniae and Its Content Comparison in Different Processed Products

作　　者：张彩[1] 张萍[1] 司文琪 赵燕[1] 史磊[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《山东农业科学》2016年第5期127-130,共4页Shandong Agricultural Sciences

基　　金：山东中医药大学2015年度大学生研究训练计划(SRT)项目(201510441011)

摘　　要：为建立怀地黄中地黄苷D的提取工艺,并比较不同炮制品中地黄苷D的含量,采用单因素实验法优选怀地黄中地黄苷D的提取工艺;采用高效液相色谱法测定怀地黄不同炮制品中地黄苷D的含量。结果表明:怀地黄中地黄苷D的最佳提取工艺条件为60%甲醇加热回流提取2 h,该工艺稳定可行,地黄苷D提取率较高;生地黄中地黄苷D的含量为0.250%,比熟地黄(0.208%)高。To establish the extraction process of rehmannioside D in Radx Rehmanniae,and compare its content in different processed products,the single factor experiments were conducted to optimize the extraction process,and the HPLC method was adopted to detect the rehmannioside D content. The results showed that the optimal extraction conditions were heating reflux extraction for 2 hours with 60% of methanol,which was stable and reliable with higher extraction rate. The content of rehmannioside D in Radix Rehmanniae was0. 250%,which was higher than that in Radix Rehmanniae Praeparata（ 0. 208%）.

关 键 词：怀地黄 地黄苷D 高效液相色谱 

分 类 号：S567.23[农业科学—中草药栽培]