血管内皮细胞生长因子可溶性受体1与慢性肾小球肾炎相关性研究  

Effect of soluble fms?like tyrosine kinase receptor 1(sFlt?1) on chronic glomerulonephritis

作　　者：孙立[1] 闫冬[2] 原泉[3] 徐天华[1] 姚丽[1] 冯江敏[1] 马健飞[1] 王力宁[1] 

机构地区：[1]中国医科大学附属第一医院肾内科,辽宁沈阳110001 [2]沈阳市红十字会医院肾内科,辽宁沈阳110001 [3]中国医科大学附属盛京医院骨外科,辽宁沈阳110001

出　　处：《中国实用内科杂志》2016年第6期469-471,共3页Chinese Journal of Practical Internal Medicine

基　　金：辽宁省社会发展攻关计划(2012225094);辽宁省高等学校优秀人才支持计划(LJQ2013086);沈阳市科技计划项目(F15-199-1-51)

摘　　要：目的检测慢性肾小球肾炎患者血、尿中可溶性血管内皮细胞生长因子受体1(sFlt-l)的水平,探讨sFlt-1在肾脏疾病中的意义。方法以2014年6月至2014年12月中国医科大学附属第一医院肾内科住院的34例肾小球肾炎患者为研究对象,根据24 h尿蛋白定量将患者分为非肾病范围蛋白尿组和肾病范围蛋白尿组。同时收集10名健康体检者作为对照组。采用酶联免疫吸附试验(ELISA)检测其血、尿中s Flt-1水平,并分析血、尿sFlt-1与24 h尿蛋白定量的相关性。结果对照组、非肾病范围蛋白尿组和肾病范围蛋白尿组患者血sFlt-1分别为(8.61±2.12)μg/L、(32.67±7.06)μg/L、(30.65±8.84)μg/L,尿s Flt-1分别为(6.66±2.37)μg/L、(29.54±5.64)μg/L、(27.41±6.79)μg/L。与对照组相比,非肾病范围蛋白尿组和肾病范围蛋白尿组患者血、尿sFlt-1表达水平明显增高,差异有统计学意义(P<0.05);非肾病范围蛋白尿组和肾病范围蛋白尿组组间比较,血、尿s Flt-1表达水平差异无统计学意义(P>0.05)。血sFlt-1表达水平与24 h尿蛋白定量无明显相关性(r=-0.029,P>0.05);尿sFlt-1的表达与24 h尿蛋白定量无明显相关性(r=0.019,P>0.05)。结论 sFlt-1在慢性肾小球肾炎发生、发展中发挥一定作用,检测sFlt-1水平并拮抗其生物学作用在慢性肾小球肾炎的诊疗中可能具有重要的意义。Objective To study the levels ofsoluble fins-like tyrosine kinase receptor 1 （sFlt-1） in the blood and urine samples ofpatients with chronic glomerulonephritis （CGN） and the correlation between sFlt- 1 and 24-hour urinary protein excretion. Methods A total of 34 patients with CGN were included in the study; along with 10 healthy volunteer controls. The patients were divided into two groups according to the 24h urinary protein excretion： a nephritic proteinuria group and a non-nephrotic proteinuria group. The sFlt-1 levels were measured by ELISA. The association between sFlt-1 levels and 24h urinary protein excretion was analyzed. Results The sFlt- i of levels in the blood in control group, nephrotic proteinuria group and the nonnephrotic proteinuria group respectivly （8.61±2.12）μg/ L, （32.67±7.06）μg/L, （30.65＋8.84）μg/L, sFlt-1 levels in the urine samples is seperately （6.66±2.37）μg/L, （29.54±5.64）μg/L, （27.41±6.79）μg/L. Compared to the control group, the sFlt-1 levels in the blood and urine samples were increased significantly in the nephrotic proteinuria group and the nonnephrotic proteinuria group （P〈0.O01）. Comparing the sFlt-1 levels between the nephrotic proteinuria group and the non- nephrotic proteinuria group, there was no significant difference （P〉0.05）. There was no linear correlation between sFlt-1 levels and 24 h urinary protein excretion（r=0.029 and 0.019, P〉0.05）. Conclusion sFlt-1 may play a role in the development of CGN. To detect the sFlt- 1 levels and block its biological effects might be of great importance in the treatment of CGN.

关 键 词：血管内皮细胞生长因子 可溶性血管内皮细胞生长因子受体1 肾小球肾炎 

分 类 号：R692[医药卫生—泌尿科学]