荧光猝灭法测定六偏磷酸根离子的浓度  

On a New Turn-off Fluorescence Probe Based on Acridine Orange for Detection of Sodium Hexametaphosphate in Beverages

作　　者：王强[1] 赵恒新[2] 王健[1] 黄承志[1,2] 

机构地区：[1]西南大学药学院,重庆400715 [2]西南大学化学化工学院,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2016年第5期13-17,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(21035005)

摘　　要：基于阳离子荧光染料吖啶橙(AO)与负电性的六偏磷酸根离子(SHMP)之间的静电作用,建立了一种测定SHMP浓度的荧光分析法.在pH=7.2的BR缓冲溶液中,SHMP能够有效猝灭AO在550nm处的荧光,且荧光强度的变化与SHMP的浓度在一定范围内呈良好的线性关系,相关系数r=0.996 6,线性范围为8.0×10^(-7)~1.12×10^(-5 )mol/L.进一步研究表明,SHMP对AO荧光的猝灭过程为静态猝灭.该方法操作简单、快速,线性范围宽,适用于饮料中SHMP添加剂浓度的测定.Based on the electrostatic interaction of cationic fluorescent dye acridine orange（AO）and negatively charged hexametaphosphate（SHMP）,a fluorescence analysis method for the determination of SHMP has been established.In BR buffer（pH=7.2）,SHMP could effectively quench the fluorescence of AO at 550 nm in the manner of static quenching.In the range of 8.0×10^-7-1.12×10^-5 mol/L SHMP,the fluorescence change of AO presented a linear relationship with the concentration of SHMP with the correlation coefficient of 0.996 6.Compared with other methods,this method is simple and fast with a wide concentration range for SHMP analysis.The results for actual sample test suggested that the established method could be applied to the determination of SHMP in beverages.

关 键 词：吖啶橙 六偏磷酸钠 荧光猝灭 

分 类 号：O657.39[理学—分析化学]