甘蓝类蔬菜根肿病抗性鉴定  

Study on clubroot resistance of different Brassica crops

作　　者：马丹丹[1,2] 庞胜群[1] 丁云花[2] 简元才[2] 耿丽华[2] 

机构地区：[1]石河子大学农学院,新疆石河子832000 [2]北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质刨制重点实验室,北京100097

出　　处：《石河子大学学报（自然科学版）》2016年第2期164-169,共6页Journal of Shihezi University(Natural Science)

基　　金：公益性行业(农业)科研专项(201003029);北京市农林科学院青年基金项目(YQNJJ201105);北京市农林科学院科技创新能力建设专项(KJCX20140111)

摘　　要：为了探讨甘蓝类蔬菜对根肿病的抗性,采用室内人工注射法和分子标记辅助选择法,对94个品种进行根肿病抗性的鉴定。结果表明:室内鉴定中有24个高抗品种,63个抗病品种和7个耐病品种;24个高抗材料都含有1个抗病主效基因CRa。此外,甘蓝高抗品种占试验材料的16.4%;黑芥-花椰菜高抗占试验材料的57.9%;花椰菜高抗品种占试验材料的25.0%,结果显示,同一类蔬菜对根肿病菌的抗病性反应存在差异,黑芥-花椰菜的抗性表现明显高于甘蓝和花椰菜,说明来自黑芥的根肿病抗性比甘蓝或花椰菜更强。This experiment explored clubroot resistance of 94 varieties of Brassica crops by adopting the artificial injection method and the molecular marker-assisted selection. The 94 varieties of Brassica crops were divided into three types, including24 high resistant varieties, 63 resistant varieties and 7 tolerance varieties by injection identification and further identification by the molecular marker-assisted selection. The results showed that all the 24 high resistant varieties have an identical gene CRa which plays major function in clubroot resistance. Among cabbage, black mustard-cauliflower and cauliflower, the high resistant varieties were 16.4%, 57.9% and 25.0% respectively, which indicated that the levels of clubroot resistance of different varieties of Brassica crops vary from each other. The black mustard-cauliflower varieties have a significantly higher clubroot resistance performance than both cabbage and cauliflower, which illustrates that the black mustard varieties have a stronger clubroot resistance either than cabbage or cauliflower.

关 键 词：芸薹属作物 根肿病 分子标记 抗性鉴定 

分 类 号：S635[农业科学—蔬菜学]