甘遂半夏汤加减甘遂及不同品种甘草对腹水模型大鼠心肝肾功能的影响  

Comparing the effect of Gansui Banxia decoction plus or reduce kansui and varieties of glycyrrhiza on heart,liver and kidney function of ascites model rat

作　　者：郭岩松[1] 柳海艳[2] 钟赣生[2] 许皖[1] 张建美[1] 刘珍清[3] 修琳琳[1] 王思睿[1] 

机构地区：[1]北京中医药大学基础医学院,100029 [2]北京中医药大学基础医学院方药系,100029 [3]北京中医药大学科研实验中心,100029

出　　处：《环球中医药》2016年第5期527-533,共7页Global Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(2011CB505306);北京中医药大学2016年度基本科研业务费项目(中青年教师类)(2016-JYB-JSMS-005)

摘　　要：目的通过分析含不同品种甘草的甘遂半夏汤加减反药组合对腹水模型大鼠的心肝肾脏功能指标的变化,探讨甘草品种对甘遂甘草配伍"反"或"不反"的影响。方法 Wistar大鼠220只随机分成11组,空白组、模型组、阳性药组、全方炙甘草组、全方胀果组、全方光果组、去遂炙甘草组、去遂胀果组、去遂光果组、去草组、去草遂组,除空白组和模型组外连续给药10天,然后腹主动脉取血,离心,检测心肝肾功能相关指标。分析方法选择方差分析,方差齐采用LSD方法,方差不齐采用Dunnet T3分析方法。结果 (1)在肝功能中TP和ALB合成方面,全方(炙胀果甘草)组的药效相对优于全方(炙光果甘草)组和全方(炙甘草)组,且三个全方组去掉一味反药或两味反药组在促进由于Walker-256细胞造成的大鼠肝脏ALB和TP合成降低方面的疗效优于三个全方组。(2)含甘遂与不同品种甘草反药组合的甘遂半夏汤全方在对Walker-256细胞造成的大鼠心功能、肾功能和肝功能中AST的含量、ALT的含量、AST/ALT比值和ALP含量变化方面无明显影响。结论甘草、光果甘草和胀果甘草这三个不同品种的甘草对甘遂甘草反药组合在心功能、肾功能和肝功能中AST的含量、ALT的含量、AST/ALT比值和ALP含量方面无明显影响,但在改善由于Walker-256细胞造成的大鼠肝脏蛋白合成异常方面,胀果甘草优于光果甘草和甘草。Objective To analyze the changes of varieties glycyrrhiza in modified Gansui Banxia decoction on heart liver and kidney function of malignant ascites rat model,and to explore the effects ofglycyrrhiza varieties on the incompatible herb. Methods 220 Wistar rats were randomly divided into blank group,model group,positive group,the complete formula with Glycyrrhiza uralensis Fisch. group,the complete formula with Glycyrrhiza inflata Bat. group,the complete formula with Glycyrrhiza glabra L.group,the Glycyrrhiza uralensis Fisch. without kansui group,the Glycyrrhiza inflata Bat. without kansui group,the Glycyrrhiza glabra L. without kansui group,Gansui Banxia decoction without glycyrrhiza group,Gansui Banxia decoction without glycyrrhiza and kansui group,except the blank group and model group,other groups were intragastric administration for 10 days,the blood simple was collected to detect relevant indicators. The single factor ANOVA and Dunnet T3 analytical method was used for statistics. Results The effect of TP and ALB synthesis in the complete formula with Glycyrrhiza inflata Bat. group was better than that in the complete formula with Glycyrrhiza glabra L. group and Glycyrrhiza uralensis group,and the whole prescription was better than modified prescription. There had no obvious effect on AST,ALT,AST /ALT and ALP in the Gansui Banxia decoction with varieties glycyrrhiza. Conclusion There had no obvious effect on AST,ALT,AST / ALT and ALP in the Gansui Banxia decoction with varieties glycyrrhiza,but the improvement action of Glycyrrhiza inflata Bat. in protein synthesis was better than Glycyrrhiza glabra L.and Licorice.

关 键 词：十八反 甘遂甘草反药组合 甘草 光果甘草 胀果甘草 

分 类 号：R285.5[医药卫生—中药学]