甘草地上部分脂溶性总黄酮抗氧化活性研究  

Evaluation of the antioxidant activity of fat-soluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch

作　　者：康雪芳 李红丽[1] 王文全[1,2,3] 侯俊玲[1,3] 

机构地区：[1]北京中医药大学中药学院,100102 [2]中国医学科学院药用植物研究所 [3]中药材规范化生产教育部工程研究中心

出　　处：《环球中医药》2016年第5期567-570,共4页Global Traditional Chinese Medicine

基　　金：国家“重大新药创制”科技重大专项(2009ZX09308-002);国家中医药管理局行业专项(201107009-03)

摘　　要：目的建立甘草地上部分脂溶性成分高效液相指纹图谱,采用DPPH自由基清除能力、还原力、羟基自由基清除能力评价甘草地上部分脂溶性总黄酮抗氧化活性。方法建立甘草地上部分脂溶性黄酮类成分高效液相指纹图谱,色谱条件：岛津Wonda Sil-C18分析柱（4.6 mm×250 mm,5μm）,乙腈-0.1%磷酸-水溶液梯度洗脱,体积流量1 m L/min,柱温35℃,检测波长190-400 nm。通过体外抗氧化活性法测定甘草地上部分脂溶性总黄酮的清除DPPH自由基能力、还原能力及清除羟基自由基能力。结果甘草地上部分脂溶性黄酮类成分有良好的清除DPPH自由基活性、清除羟基自由基能力及还原能力。结论建立的HPLC-DAD指纹图谱方法能较好的反应甘草地上部分脂溶性黄酮类成分,甘草地上部分脂溶性总黄酮有较强的抗氧化能力。Objective To establish the HPLC fingerprint of fat-soluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch.,the DPPH radical scavenging,reducing antioxidant power assay,hydroxyl free radical scavenging capacity was used to evaluate antioxidant activity of fat-soluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch.. Method HPLC fingerprint of the aerial parts of the fat-soluble flavonoids of Glycyrrhiza uralensis Fisch. was established,HPLC fingerprint chromatographic conditions： DIKMA Wonda Sil-C18 analytical column（ 4. 6 × 250 mm,5μm）,acetonitrile- 0. 1% phosphoric acid-water gradient,the volumetric flow rate was 1m L / min,column temperature was 35℃,detection wavelength was 190- 400 nm. In vitro antioxidant activity was measured by DPPH radical scavenging method,reducing power and scavenging hydroxyl radical assay. Results Fat-soluble flavonoids in aerial parts of Glycyrrhiza ura-lensis Fisch. had a strong antioxidant capacity. Conclusion HPLC-DAD fingerprint method can show fatsoluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch.. It has good DPPH radical scavenging activity,scavenging hydroxyl free radical activity and reducing power.

关 键 词：甘草地上部分 脂溶性总黄酮 HPLC-DAD 

分 类 号：R284[医药卫生—中药学]