LPS及TGF-β对巨噬细胞中Tim-3表达的影响及其机制的初步探索  

Roles and mechanisms of LPS and TGF-β in regulating the expression of Tim-3 in macrophage

作　　者：王位[1,2] 董忱[3] 史青竹 侯春梅[1] 石新慧[1] 李葛[1] 窦帅杰 黎燕[1] 沈倍奋[1] 马远方[2] 韩根成[1] 

机构地区：[1]军事医学科学院基础医学研究所天然免疫研究室,北京100850 [2]河南大学医学院细胞与分子免疫实验室,河南开封475001 [3]济南军区青岛第二疗养院,山东青岛266071

出　　处：《军事医学》2016年第5期387-391,共5页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81471540);北京市自然科学基金重点资助项目(7141007)

摘　　要：目的探讨促炎因子脂多糖(lipopolysaccharide,LPS)和抑炎因子转化生长因子-β(transforming growth factor-beta,TGF-β)对巨噬细胞中T细胞免疫球蛋白黏蛋白-3(T-cell immunoglobulin and mucin domain-containing protein3,Tim-3)表达的影响,并初步探索其影响机制。方法使用LPS和TGF-β分别刺激小鼠源巨噬细胞系RAW264.7、人源巨噬细胞系U937,在基因水平和蛋白水平分别检测巨噬细胞中Tim-3的表达情况,同时观察巨噬细胞中金属蛋白酶ADAM10/17在基因水平的变化。结果 LPS可抑制巨噬细胞中Tim-3在基因和蛋白水平的表达,而TGF-β作用相反。LPS促进巨噬细胞中ADAM10/17在基因水平的表达,TGF-β作用正相反。结论炎症微环境中促炎介质LPS和抑炎介质TGF-β分别抑制和促进巨噬细胞中Tim-3的表达,同时,金属蛋白酶ADAM10/17可能参与LPS、TGF-β对巨噬细胞中Tim-3在蛋白水平表达的调控。Objective To explore the roles and mechanisms of lipopolysaccharide（ LPS） and transforming growth factor（ TGF）-β in regulating the expression of T-cell immunoglobulin and mucin domain protein（ Tim）-3 in macrophage.Methods The expression of Tim-3 and ADAM10 /17 in macrophage cell lines RAW264. 7 and U937 was determined,stimulated by LPS or TGF-β. Results LPS suppressed the expression of Tim-3 and promoted the expression of ADAM10 /17,but TGF-β did not. Conclusion Inflammatory mediators,LPS and TGF-β,can inhibit and promote the expression of Tim-3 in macrophage,respectively. Furthermore,metalloproteinase ADAM10 /17 may be involved in the process in which LPS and TGF-β regulate the expression of Tim-3 at the protein level under microenvironments.

关 键 词：脂多糖类 转化生长因子Β TIM-3 巨噬细胞 

分 类 号：R392[医药卫生—免疫学]