不同程度肺型氧中毒大鼠血气指标变化的实验研究  

Dynamic changes of acid-base status in pulmonary injury induced by hyperbaric oxygen in rats

作　　者：包晓辰[1] 刘平小[1] 马骏[1] 攸璞[1] 王芳芳[1] 方以群[1] 

机构地区：[1]海军医学研究所,上海200433

出　　处：《军事医学》2016年第5期409-411,416,共4页Military Medical Sciences

基　　金：海军后勤部科研基金资助项目(12A107);海军医学研究所基金资助项目(12HY11)

摘　　要：目的通过测定高压氧暴露不同时间后大鼠的血气分析值,探究肺型氧中毒时体内酸碱平衡的动态改变。方法 50只雄性SD大鼠,随机分为5组,分别暴露于2.3绝对压(ATA)、100%氧气中3、6、9、12 h,空气暴露组作为对照。出舱后观测大鼠生存率、行为改变、肺组织湿干比。抽取股动脉血,行血气分析。结果在2.3 ATA、100%氧气暴露后大鼠体内酸碱平衡呈现动态改变。与空气暴露组相比,血气分析值在暴露后3 h无明显改变;暴露6及9 h组与暴露3 h相比有显著的统计学差异;而暴露12 h组与6及9 h相比又有显著性差异。肺湿干比的结果显示,暴露3 h组与空气暴露组相比无明显差异,暴露6、9及12 h组显著上调,12 h组与3、6及9 h组相比明显增高。结论高压氧导致的肺型氧中毒动物体内酸碱平衡紊乱,从代偿期向失代偿碱中毒过渡,最后发展至失代偿性酸中毒。血气分析值在判断肺型氧中毒的疾病发展阶段具有重要的指导意义。Objective To find out about the changes of acid-base status in pulmonary oxygen toxicity. Methods Fifty male SD rats were randomly divided into 5 groups,exposed to 2. 3ATA（ atmosphere absolute） and 100% oxygen for 3,6,9and 12 h,respectively. Rats exposed to air were used as control. After exposure,the mortality and behavior of rats were observed. The wet / dry weight ratio of lungs was defined. The arterial blood was collected from the femoral artery and used to analyze the blood gas. Results The acid-base status in rats exposed to 2. 3ATA,and 100% oxygen presented a dynamic change. The primary status was in the compensatory period when primary respiratory acidosis were mixed with compensated metabolic alkalosis. Then the status changed to decompensated alkalosis and developed to decompensated acidosis in the end. As compared to control group,the blood gas analysis did not change in 3 h group. When compared to 3 h group,the blood gas analysis changed markedly in 6 h and 9 h groups. The same trend was also observed in 12 h group when compared to 6 h and 9 h groups. The result of lung wet / dry ratio was increased significantly in 6 h,9 h and 12 h groups when compared to control group,while these changes were not so obvious in 3 h group. The lung wet / dry ratio in 12 h group was also significantly different when compared to 3 h,6 h and 9 h groups. Conclusion The acid-base status in pulmonary oxygen toxic rats presents a dynamic change,which is in the compensatory period first,then turns to decompensated alkalosis and ends up with decompensated acidosis status. Blood gas analysis is a useful method to monitor the development of pulmonary oxygen toxicity.

关 键 词：肺型氧中毒 血气分析 酸碱平衡 

分 类 号：R459.6[医药卫生—治疗学] R595[医药卫生—临床医学]