扶正化瘀胶囊对二甲基亚硝胺诱导的肝纤维化大鼠血清代谢组学的影响  

Effect of Fuzheng Huayu capsule on serum metabolomics in rats with liver fibrosis induced by dimethylnitrosamine

作　　者：高国瑗 郑希元[2] 彭渊[2] 陶艳艳[2] 刘平[2,3] 杨涛[4] 刘成海[1,2,3] 

机构地区：[1]华东理工大学药学院,上海200237 [2]上海中医药大学附属曙光医院肝病研究所,上海201203 [3]上海高校中医内科学E研究院,上海201203 [4]上海中医药大学附属曙光医院心血管研究所,上海201203

出　　处：《中国中药杂志》2016年第9期1725-1731,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81530101);国家国际科技合作专项(2014DFA31440);艾滋病和病毒性肝炎等重大传染病防治专项(2014ZX10005001);上海市医学领军人才计划项目(LJ10005);上海中医药事业发展三年行动计划项目(ZY3-CCCX-2-1003)

摘　　要：探讨扶正化瘀胶囊(Fuzheng Huayu capsules,FHC)对二甲基亚硝胺(dimethylnitrosamine,DMN)诱导的肝纤维化模型大鼠血清代谢组学的影响。应用液质联用技术分别建立对照组、模型组和FHC组的大鼠血清代谢图谱,并定量分析各组内源性代谢物——氨基酸和胆汁酸水平。实验结果表明,FHC组大鼠的血清代谢指纹谱与模型组存在显著差异,采用主成分分析(PCA)和偏最小二乘法(PLS-DA)初步鉴定了潜在5种溶血磷脂胆碱类生物标记物。再通过定量分析各组大鼠血清氨基酸和胆汁酸的水平,发现FHC治疗4周后血清中失调的14种氨基酸和5种胆汁酸水平明显有回归正常水平的趋势。由此可见,FHC抗肝纤维化作用机制可能涉及到溶血磷脂胆碱类、氨基酸和胆汁酸的代谢过程。To investigate the effect of Fuzheng Huayu capsule（ FHC） on serum metabolomics in rats with liver fibrosis induced by dimethylnitrosamine（ DMN）. The metabolic profiles of rat serum of normal group,model group,and FHC group were established by liquid chromatography-mass spectrometry technology. Furthermore,the levels of endogenous metabolites such as amino acids and bile acids were measured in each group. The results showed that there were significant differences in the serum metabolic fingerprints between the FHC group and the model group. Moreover,5 potential lysophosphatidylcholines biomarkers were identified by using principal component analysis（ PCA） and partial least squares discriminant analysis（ PLS-DA）. Quantitative analysis of amino acids and bile acids in serum of rats showed that 14 kinds of amino acids and 5 kinds of bile acids returned to normal levels after four weeks of FHC treatment. In conclusion,the anti-hepatic fibrosis mechanisms of FHC may be related to the metabolic process of lysophosphatidylcholines,amino acids and bile acids.

关 键 词：扶正化瘀 肝纤维化 代谢组学 液质联用 生物标记物 

分 类 号：R285.5[医药卫生—中药学]