植物防御信号物质JA/SA对桃蚜解毒酶谷胱甘肽-S-转移酶及唾液腺基因C002表达诱导反应  

Effects of Plant Defense Signal Molecules Jasmonic Acid and Salicylic Acid on the Expression of Detoxification Enzyme Glutathione S-transferases and Salivary Protein C002 in Myzus persicae

作　　者：张勇[1] 范佳[1] 赵兴延[1,2] 刘勇[2] 孙京瑞[1] Frederic Francis 陈巨莲[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]山东农业大学植物保护学院,泰安271000 [3]Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, Gembloux B-5030, Belgium

出　　处：《中国科学：生命科学》2016年第5期665-672,共8页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:30971920,31371946);中国农业科学院科技创新工程资助

摘　　要：茉莉酸(JA)与水杨酸(SA)为植物体内重要的防御反应信号物质,在植株受到机械损伤或病虫害侵害时可作为信号物质,激活下游相关防御反应.为应对植株的防御反应,昆虫通常通过提高自身相关解毒酶活性或分泌唾液蛋白调节寄主防御反应以增强对寄主植株适应性.本研究以桃蚜(Myzus persicae(Sulzer))体内的重要解毒酶谷胱甘肽-S-转移酶(GST)的sigma型及唾液腺特异表达基因C002为研究对象,分别以含有5 mmol/L JA或10 mmol/L SA的人工饲料直接饲喂桃蚜,在不同的取食时间点收集蚜虫,通过荧光定量PCR检测JA或SA对目的基因sigma GST及C002表达诱导反应.结果发现,当桃蚜直接取食含有JA或SA的人工饲料后,sigma GST及C002表达量都显著升高.表明,桃蚜可直接利用寄主植株防御反应信号物质JA或SA,提高体内相关解毒酶或者唾液蛋白基因表达量,以提高对寄主防御的适应性.为进一步开展桃蚜对植物防御反应适应性研究提供新的思路.Jasmonic acid（JA） and salicylic acid（SA） are important phytohormones for activating plant defense responses when plants suffer from mechanical damage or pest infestation. In response to plant defense responses, insects usually increase the detoxification enzyme activity or secrete some saliva proteins into plants to modulate host cell processes to promote their adaptation. In this study, we used real-time q PCR to detect the relative gene expression of detoxification enzyme named glutathione S-transferases（sigma GST） and salivary gland-specific protein C002 in Myzus persicae after feeding with 5mM JA or 10 mM SA through artificial diet. The results showed that the relative expression of sigma GST and C002 in M. persicae increased significantly after JA and SA treatments. The results revealed that M. persicae can use JA and SA as cues to up-regulate gene expression of related detoxification enzyme and saliva protein. Our results provided new insights into the research on the mechanism of M. persicae adaptation to host plant resistance.

关 键 词：桃蚜 茉莉酸 水杨酸 sigma型谷胱甘肽-S-转移酶 唾液蛋白C002 

分 类 号：S432.2[农业科学—植物病理学]