丹参酮ⅡA对人膀胱癌裸鼠移植瘤微血管密度、碱性成纤维细胞生长因子、血管内皮生长因子表达的影响  

Effect of tanshinone Ⅱ A on microvessel density and the expression of basic fibroblast growth factor, vascular endothelial growth factor of human bladder cancer transplanted tumor in nude mice

作　　者：王进[1] 李森[1] 张友朋[1] 曾汉青[1] 朱朝辉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院泌尿外科,武汉430022

出　　处：《中华实验外科杂志》2016年第5期1275-1277,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察丹参酮ⅡA对人膀胱癌裸鼠移植瘤微血管密度、碱性成纤维细胞生长因子、血管内皮生长因子表达的影响.方法 选择BALB/c裸鼠30只为研究对象,建立膀胱癌移植瘤模型后分为模型组、塞替哌组、丹参酮ⅡA组,分别给予生理盐水、塞替哌、丹参酮ⅡA干预,实验4周后处死,观察肿瘤生长情况、血管新生情况、凋亡蛋白的表达量.结果 4周后,丹参酮ⅡA组小鼠肿瘤重量、膀胱湿重指数明显高于模型组、塞替哌组,抑瘤率明显高于塞替哌组[（0.62±0.07）g比（0.91 ±0.09） g,（0.68±0.00） mg/g比（0.90±0.08） mg/g,53.38比31.58,t =4.243～8.981,P＜0.05）;丹参酮ⅡA组微血管密度（MVD）、碱性成纤维细胞生长因子（bFGF）、血管内皮生长因子（VEGF）的mRNA明显高于模型组、塞替哌组（8.45±0.75比13.06±1.65,35.52±4.29比65.14±7.21,41.39±4.77比68.32±7.24,t =7.601～15.732,P＜0.05）;丹参酮ⅡA组肿瘤组织促凋亡蛋白基因[p53、B细胞淋巴瘤/白血病-2相关X蛋白（bax）]表达量明显高于模型组、塞替哌组（1.25±0.26比0.64 ±0.07,0.86±0.12比0.67 ±0.08）,抗凋亡蛋白基因[B细胞淋巴瘤/白血病-2（bcl-2）、生存素（Survivin）]表达量明显低于模型组、塞替哌组（0.41±0.04比0.70±0.09,0.38±0.04比0.85±0.09）（t=2.821 ～ 16.149,P＜0.05）.结论 丹参酮ⅡA能够抑制人膀胱癌裸鼠移植瘤的生长,减少血管密度以及促血管新生分子的生成,诱导细胞凋亡.Objective To study the effect of tanshinone Ⅱ A on microvessel density （MVD） and the expression of basic fibroblast growth factor （bFGF） and vascular endothelial growth factor （VEGF） of human bladder cancer transplanted tumor in nude mice.Methods Thirty-six BALB/c nude mice were collected,and divided into model group,thiotepa group,and tanshinone Ⅱ A group after bladder cancer xenograft models were established.Four weeks later,the tumor growth,angiogenesis,and expression of apoptosis proteins were observed.Results At 4th week,bladder tumor weight,and wet weight index were significantly higher in tanshinone Ⅱ A group than in model group and thiotepa group,and the inhibitory rate in tanshinone Ⅱ A group was significantly higher than that of thiotepa group [（0.62 ± 0.07） g vs.（0.91 ±0.09） g,（0.68 ±0.00） mg/g vs.（0.90 ±0.08） mg/g,and 53.38 vs.31.58,t =4.243-8.981,P 〈 0.05].MVD,bFGF and VEGF mRNA expression levels in tanshinone Ⅱ A group were significantly lower than those in model group,and thiotepa group [（8.45 ±0.75 vs.13.06 ± 1.65）,（35.52 ± 4.29 vs.65.14 ±7.21）,and （41.39 ±4.77 vs.68.32 ±7.24）] （t =7.601-15.732,P 〈0.05）.The expression levels of p53,and B cell lymphoma/leukemia-2 associated X protein （bax） in tanshinone Ⅱ A group were significantly higher than those in model group and thiotepa group （1.25 ± 0.26 vs.0.64 ± 0.07,0.86 ±0.12 vs.0.67 ±0.08）,and those of B cell lymphoma/leukemia-2 （bcl-2）,and Survivin were significantly lower than those in model group and thiotepa group （0.41 ±0.04 vs.0.70 ±0.09,and 0.38 ± 0.04 vs.0.85 ± 0.09,t =2.821-16.149,P 〈 0.05）.Conclusion Tanshinone Ⅱ A can inhibit the growth of transplanted tumor of human bladder cancer in nude mice,reduce the density of blood vessels and the production of pro-angiogenesis molecules,and induce cell apoptosis.

关 键 词：膀胱癌 丹参酮ⅡA 移植瘤 血管新生 凋亡蛋白 

分 类 号：R285.5[医药卫生—中药学]