紫茉莉乙醇提取物对二斑叶螨生物酶活性的影响  

Effect of Mirabilis jalapa Ethanol Extracts on Enzymes of Tetranychus urticae

作　　者：赵赫南 雷廷[1] 李冰[1] 曹永昶 任航[1] 韩俊艳[1] 

机构地区：[1]沈阳大学城市有害生物治理与生态安全辽宁省重点实验室,辽宁沈阳110044

出　　处：《沈阳大学学报（自然科学版）》2016年第3期198-202,共5页Journal of Shenyang University：Natural Science

基　　金：辽宁省自然科学基金资助项目(2014020100)

摘　　要：为探讨紫茉莉乙醇提取物杀二斑叶螨作用机制,研究了紫茉莉乙醇提取物(MEE)对几种生物酶活性的影响.以MEE最大可耐受剂量1.2mg/mL采用触杀法处理雌成螨,分别在处理后4、8、12、16、20、24h后测定酶活性变化.处理组螨的蛋白酶活性、酯酶活性及Na^+,K^+-ATP酶活性均呈持续下降状态,谷胱甘肽巯基转移酶(GST)活性呈先上升后下降状态,在24h内活性均高于对照组.试验结果表明,MEE对二斑叶螨的几种酶活性产生影响,提示其杀螨作用机制与这些酶有关.For elucidating the mechanisms of Mirabilis jalapa ethanol extracts(MEE)killing Tetranychus urticae,effects of MEE on enzyme activities of Tetranychus urticae were determined.The maximum tolerable dose of 1.2 mg/mL MEE were used to treat female adult Tetranychus urticae,the enzyme activities were measured after 4,8,12,16,20 and 24h,respectively.The enzyme activities of proteinase,Na^+,K^+-ATPase and esterase decreased in all the checkpoints,and glutathione S-transferase activity increased firstly and decreased latterly.The results demonstrated that MEE affected the enzyme activities of Tetranychus urticae,which may involve in the mechanisms of MEE killing Tetranychus urticae.

关 键 词：生物农药 杀螨剂 谷胱甘肽巯基转移酶 Na+ K+ATP酶 蛋白酶 

分 类 号：S482.39[农业科学—农药学]