HPLC同时测定大血藤中绿原酸、咖啡酸和香草酸的含量  

Simultaneous Determination of Chlorogenic Acid, Caffeic Acid and Vanillic Acid in Sargentodoxae Caulis by HPLC

作　　者：荆彦民 马瑞丽[1,2] 

机构地区：[1]定西市农业科学研究院,甘肃定西743000 [2]江苏大学药学院,江苏镇江212013

出　　处：《中国现代应用药学》2016年第5期592-595,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：甘肃省定西市道地中药材产业化推广及惠民示范工程(S2013GMG100004)

摘　　要：目的建立同时测定大血藤各部位绿原酸、咖啡酸和香草酸含量的高效液相色谱方法。方法色谱柱为Kromasil C18柱(4.6 mm×250 mm,4.5μm),流动相:A为1%冰醋酸,B为甲醇,以A∶B=68∶32等度洗脱;柱温:30℃;流速:1 m L·min?1;检测波长:327 nm;进样量:20μL。结果在测定样品中,绿原酸和咖啡酸在乙酸乙酯部位含量最高,分别为4.52%,0.11%,香草酸在二氯甲烷部位含量最高,为5.8%。结论该方法简便、准确、重复性高,可用于大血藤中绿原酸、咖啡酸、香草酸含量的测定。OBJECTIVE To develop an HPLC method for simultaneous determination of chlorogenic acid, caffeic acid and vanillic acid in each fraction of Sargentodoxae Caulis. METHODS The analysis was carried on a column of Kromasil C18（4.6 mm×250 mm, 4.5 μm）. Isocratic elution, mobile phase consisted of solvent A（1%, solution of HAc in water） and solvent B（methanol） at the ratio of 68∶32, the flow rate was 1.0 m L.min^-1, the injection volume was 20 μL, the detection wavelength was 327 nm, the column temperature was at 30 ℃. RESULTS Ethyl acetate fraction had the highest concentration of chlorogenic acid（4.52%） and caffeic acid（0.11%） and dichloromethane fraction possessed the highest concentration of vanillic acid of 5.8%. CONCLUSION This method is simple, accurate, rapid and suitable for the usual quality control of Sargentodoxae Caulis.

关 键 词：高效液相色谱法 大血藤 绿原酸 咖啡酸 香草酸 

分 类 号：R917.101[医药卫生—药物分析学]