HPLC同时测定宣肺止咳合剂3种指标成分的含量  

Study on Simultaneous Determination of Three Main Marker Components in Xuanfeizhike Mixture by HPLC

作　　者：王莹[1] 杜静[1] 

机构地区：[1]杭州市红十字会医院,杭州310003

出　　处：《中国现代应用药学》2016年第5期599-601,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：杭州市科学技术委员会项目(20130633B25);浙江省中西医结合学会临床药学科研基金(2013LYD005)

摘　　要：目的建立反相高效液相色谱法同时测定宣肺止咳合剂中葛根素、大豆苷、甘草酸的含量。方法采用Agilent Zorbax SB-C18（4.6 mm×250 mm,5μm）色谱柱,检测波长：254 nm,以乙腈-0.1%甲酸水为流动相,梯度洗脱,柱温为30℃。结果葛根素、大豆苷、甘草酸浓度分别在99.14-991.4,22.50-225.0,22.60-226.0μg·m L^-1内与峰面积呈良好的线性关系（r≥0.999 8）,平均回收率分别为99.2%,98.8%,99.3%。结论该方法简便、准确、重复性好,为宣肺止咳合剂的质量评价与质量控制提供了科学依据。OBJECTIVE To establish an HPLC method for simultaneous determination of puerarin, daidzein and glycyrrhizic acid in Xuanfeizhike mixture. METHODS Isolation of sample was achieved on an Agilent Zorbax SB-C18（4.6 mm× 250 mm, 5 μm）, under the 254 nm detection wavelength, gradient eluted by acetonitrile-0.1% formic acid, and the column temperature was maintained at 30 ℃. RESULTS The linearity ranges of puerarin, daidzein and glycyrrhizic acid were 99.14-991.4, 22.50-225.0, 22.60-226.0 μg·m L^-1, respectively（r≥0.999 8）. The average recoveries（n=9） of them were 99.2%, 98.9%, 99.3%. CONCLUSION The method is simple, accurate and has a good repeatability. The study provides scientific foundation for quality evaluation and quality control standards for Xuanfeizhike mixture.

关 键 词：宣肺止咳合剂 高效液相色谱法 葛根素 大豆苷 甘草酸 含量测定 

分 类 号：R284.1[医药卫生—中药学] R917.101[医药卫生—中医学]