全蝎水提物对巨噬细胞活化作用研究  

Studies on macrophage activations of BMWE in vitro

作　　者：刘芬[1] 侯睿[1] 费巧玲[1] 高源[1] 蔡润兰[1] 陶燕蓉[2] 王峥涛[3] 齐云[1] 

机构地区：[1]中国医学科学院&北京协和医学院药用植物研究所药理毒理中心,北京100193 [2]北京中医药大学东直门医院,北京100029 [3]上海中医药大学中药研究所,上海201203

出　　处：《中国免疫学杂志》2016年第5期660-664,共5页Chinese Journal of Immunology

基　　金：国家科技重大专项(2014ZX09201022-006;2012ZX09301-002-001);北京自然科学基金项目(7142112)资助

摘　　要：目的:观察全蝎水提物(Buthus martensii water extract,BMWE)在体外对小鼠巨噬细胞株RAW264.7的活化作用。方法:MTT法测定细胞活性;采用FITC标记的酵母测定吞噬活性;利用Griess试剂和ELISA方法测定细胞上清NO、TNF-α和IL-6释放量。结果:BMWE在不影响细胞活力的剂量范围内可增强正常及免疫抑制的RAW264.7细胞吞噬荧光酵母能力(P<0.01),增加RAW264.7细胞NO释放量(P<0.01),促进RAW264.7细胞TNF-α和IL-6产生(P<0.01),均具有良好的量效关系。结论:BMWE可明显提高巨噬细胞吞噬功能与分泌能力,活化巨噬细胞,此作用与全蝎传统用于治疗疮疡、瘰疬,现代用于肿瘤的临床应用相关。Objective： To investigate the activation effect of Buthus martensii water extract（ BMWE） in RAW 264. 7cells. Methods： The MTT assay and FITC-conjugated yeast were used to evaluate the effects of BMWE on the viability and phagocytosis of RAW264. 7 cells,respectively. NO production in supernatant was measured by Griess reagent. IL-6 and TNF-α production of the cells induced by BMWE were measured by ELISA. Results： Cell viability analysis showed that BMWE did not affect the cell viability up to a concentration of 200 μg / ml. Pretreatment with BMWE potently enhanced phagocytic function（ P〈0. 01） in the presence or absence of dexamethasone in RAW 264. 7 cells. BMWE also significantly increased NO,TNF-α and IL-6 production（ P〈0. 01） in a dose-dependent manner. Conclusion： BMWE can activate the macrophages showing that the phagocytosis and secretion function were markedly enhanced.

关 键 词：全蝎水提物 巨噬细胞 吞噬活性 一氧化氮 白介素-6 肿瘤坏死因子-Α 

分 类 号：R392.12[医药卫生—免疫学]