含甲磺酸去铁胺低温保存液保护大鼠心脏的效果  

Efficacy of cold preservation solution containing desferrioxamine in protecting rat hearts

作　　者：伍佳莉[1] 苏松[2] 胡许平[1] 欧册华[1] 

机构地区：[1]泸州医学院附属医院麻醉科,646000 [2]泸州医学院附属医院肝胆外科

出　　处：《中华麻醉学杂志》2016年第3期325-327,共3页Chinese Journal of Anesthesiology

基　　金：四川省人事及社会保障厅归国留学人才择优资助项目（2010-1021号）;泸州医学院2011年自然科学基金重点资助项目

摘　　要：目的 评价含甲磺酸去铁胺低温保存液保护大鼠心脏的效果.方法 清洁级健康成年雄性SD大鼠,体重300～350 g.制备成功的离体心脏32个,采用随机数字表法分为2组（n=16）：对照组（C组）和甲磺酸去铁胺组（DFO组）.C组将离体心脏置于4 ℃ HTK保存液中保存6h;DFO组将甲磺酸去铁胺加入HTK保存液（甲磺酸去铁胺浓度为100 μmol/L）中保存6h.于冷保存6h时测定保存液肌酸激酶和乳酸脱氢酶活性;取心尖组织行HE染色,观察心肌组织病理学结果并行病理学损伤评分;采用硫代巴比妥酸法测定心肌组织MDA含量,Western blot法及RT-PCR法检测缺氧诱导因子1α及其mRNA表达水平.结果 与C组比较,DFO组保存液肌酸激酶和乳酸脱氢酶活性、心肌MDA含量及病理学损伤评分降低,心肌缺氧诱导因子1α及其mRNA表达上调（P＜0.05）.结论 含甲磺酸去铁胺低温保存液可有效保护大鼠心脏,其机制与上调缺氧诱导因子1α表达有关.Objective To evaluate the efficacy of cold preservation solution containing desferrioxamine （DFO） in protecting the rat hearts.Methods Pathogen-free male Sprague-Dawley rats,weighing 300-350 g,were used in the study.Thirty-two isolated rat hearts were equally and randomly divided into control group （C group） and DFO group using a random number table.Hearts of rats were stored for 6 h in 4 ℃ histidine-tryptophan-ketoglutarate （HTK） solution in group C.DFO was added to HTK solution （DFO concentration 100 μmol/L）,and hearts of rats were stored for 6 h in 4 ℃ HTK solution in group DFO.At 6 h of cold storage,creatine kinase and lactate dehydrogenase activities in the cold preservation solution were determined.Myocardial specimens were obtained from the apex,cut into sections which were stained with haematoxylin and eosin,and examined under a microscope.The pathological changes of myocardial tissues were scored.The content of malondialdehydc in myocardial tissues was determined using thiobarbitnric acid method,and hypoxia-inducible factor-1α protein and mRNA expression in myocardial tissues was detected by real-time reverse transcriptase polymerase chain reaction and Western blot.Results Compared with group C,the creatine kinase and lactate dehydrogenase activities in the cold preservation solution,malondialdehyde content in myocardial tissues,and pathological scores were significantly decreased,and the expression of hypoxia-inducible factor-1α protein and mRNA expression in myocardial tissues was significantly up-regulated in group DFO （P〈 0.05）.Conclusion Cold preservation solution containing DFO can protect the rat hearts effectively,and the mechanism is related to up-regulation of the expression of hypoxia-inducible factor-1α.

关 键 词：去铁胺 低温保存 心肌缺血 

分 类 号：R965[医药卫生—药理学]