脐带间充质干细胞移植对内毒素血症大鼠认知功能的影响  

Effect of umbilical cord mesenchymal stem cell transplantation on cognitive function in endotoxemic rats

作　　者：魏彭辉 郑强[1] 周金锋[1] 李建军[1] 

机构地区：[1]山东大学齐鲁医院麻醉科,济南市250012

出　　处：《中华麻醉学杂志》2016年第3期365-368,共4页Chinese Journal of Anesthesiology

基　　金：Shandong Province Science and Technology Program(2011GSF11801)山东省科技发展计划

摘　　要：目的 评价脐带间充质干细胞移植对内毒素血症大鼠认知功能的影响.方法 清洁级健康成年雄性Wistar大鼠72只,体重180～250 g,采用随机数字表法分为4组（n=18）：对照组（C组）、内毒素血症组（E组）、脐带间充质干细胞组（M组）和内毒素血症＋脐带间充质干细胞组（PM组）.采用Morris水迷宫实验测定大鼠认知功能,在定位航行实验的第2天水迷宫训练前4h,E组腹腔注射脂多糖100 μg/kg,C组注射等容量生理盐水,PM组腹腔注射内毒素后即刻尾静脉注射300 μl脐带间充质干细胞悬液（细胞数5＆#215;105个,液体为生理盐水）,M组尾静脉注射脐带间充质干细胞.注射后4h继续行水迷宫训练.分别在腹腔或尾静脉注射后4、24 h和5 d（T1-3）时每组随机取6只大鼠,心脏取血后,处死取海马,测定血清和海马IL-1β、IL-6和TNF-α水平.结果 与C组比较,E组在水迷宫训练第2和3天逃避潜伏期延长,穿越平台次数减少,靶象限距离百分比降低,T12时血清和海马IL-1β、IL-6和TNF-α水平升高（P＜0.05）;与E组比较,PM组在水迷宫训练第2和3天逃避潜伏期缩短,穿越平台次数增加,靶象限距离百分比升高,T1,2时血清和海马IL-1β、IL-6和TNF-α水平降低（P＜0.05）.结论 脐带间充质干细胞移植可改善内毒素血症大鼠认知功能降低.Objective To evaluate the effect of umbilical cord mesenchymal stem cell （UC-MSC） transplantation on cognitive function in endotoxemic rats.Methods Seventy-two pathogen-free male Wistar rats,aged 180-250 g,were randomly divided into 4 groups （n=18 each） using a random number table：control group （group C）,endotoxemia group （group E）,UC-MSC group （group M）,and endotoxemia＋ MSC group （group PM）.Cognitive function was assessed using Morris water maze test.At 4 h before training on 2nd day of place navigation test,lipopolysaccharide 100 μg/kg was injected intraperitoneally in group E,the equal volume of normal saline was given in group C,UC-MSC suspension 300 μl （5＆#215; 105cells,the fluid was normal saline） was injected via the tail vein immediately after intraperitoneal lipopolysaccharide in group PM,and UC-MSCs were injected via the tail vein in group M.At 4 h after injection,Morris water maze test was continued.At 4,24 h and 5 days after intraperitoneal injection or injection via the tail vein （T1-3）,6 rats were selected,and blood samples were collected from hearts.The rats were then sacrificed,and the hippocampus was removed.The levels of interleukin-lbeta （IL-1β）,IL-6 and tumor necrosis factor-alpha （TNF-α） in serum and hippocampus were determined.Results Compared with group C,the escape latency was significantly prolonged on 2nd and 3rd days of Morris water maze test,the number of crossing the platform was significantly decreased,the percentage of swimming distance in 1 st quadrant in the total swimming distance was significantly decreased,and the levels of IL-1 β,IL-6 and TNF-α in serum and hippocampus were significantly increased at T1,2 in group E （P〈0.05）.Compared with group E,the escape latency was significantly shortened on 2nd and 3rd days of Morris water maze test,the number of crossing the platform was significantly increased,the percentage of swimming distance in 1st quadrant in the total swimming distance was significantly increased,and the le

关 键 词：间质干细胞移植 内毒素血症 认知障碍 海马 

分 类 号：R459.7[医药卫生—急诊医学]