基于新疆紫草转录组的对羟基苯甲酸香叶基转移酶(PGT)基因的挖掘及生物信息学分析  

Transcriptome-based gene mining and bioinformatics analysis of p-hydroxybenzoate geranyltransferase genes in Arnebia euchroma

作　　者：刘谈[1] 吕朝耕 王升[1] 杨婉珍[1,2] 郭兰萍[1] 

机构地区：[1]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700 [2]首都医科大学中医药学院,北京100069

出　　处：《中国中药杂志》2016年第8期1422-1429,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81130070;81072989);国家科技支撑计划项目(2012BAI29B02;2012BAI28B002)

摘　　要：对羟基苯甲酸香叶基转移酶(PGT)在紫草素类化合物的生物合成途径中起重要作用。该文主要通过生物信息学的方法从新疆紫草转录组中获得6个PGT基因,并预测其编码蛋白的理化性质,进行相关基因的表达分析。结构域预测结果显示,6个PGT蛋白序列均包含Ubi A异戊烯基转移酶家族保守结构域及异戊烯焦磷酸结合位点NDxx Dxxx D和可能的芳环的结合位点GX(K/Y)ST AL;系统进化树分析显示PGT与同源的对羟基苯甲酸多异戊烯转移酶(PPT)属于2个不同的进化枝;基因表达分析表明在紫草素类化合物产量较高的新疆紫草红色高产系悬浮细胞及植株的地下部分,其PGT基因的表达量明显高于白色低产系及植株的地上部分,说明新疆紫草PGT基因的表达量与紫草素类化合物的合成呈正相关。该研究为进一步了解对羟基苯甲酸香叶基转移酶(PGT)的功能及特性奠定了基础,并为紫草素类化合物生物合成及代谢调控提供依据。The p-hydroxybenzoate geranyltransferases（ PGT） play an important role in the biosynthesis pathways of shikonin derivatives. Six PGTs were obtained from transcriptome datebase of Arnebia euchroma by using bioinformatics methods and the proteins＇ physiochemical properties they encoded were predicted. The result of protein domain prediction showed all of the six protein sequences contained the conserved domain of Ubia prenyltransferase family and possessed the motif NDxx Dxxx D for prenyldiphosphate binding and a GX（ K / Y） STAL sequence for putative aromatic ring binding. The phylogenetic tree showed that PGT and p-hydroxybenzoate polyprenyltransferase（ PPT） belonged to two different clades. The results of gene expression analyses showed that the expression levels in the red shikonin-proficient line and the overground part of A. euchroma that could produce shikonin derivatives was much higher than the white shikonin-deficient line and the underground part,which suggested a positive correlation between the expression levels of PGT genes and shikonin production. This study aims to lay a foudation for further understanding of the function and enzymatic properties of PGT and provide a basis for the biosynthesis pathways and metabolic regulation of shikonin derivatives.

关 键 词：新疆紫草 对羟基苯甲酸香叶基转移酶 生物信息学分析 实时荧光定量 

分 类 号：S567.239[农业科学—中草药栽培]