氧化苦参碱对哮喘大鼠肺组织IL-10表达的影响  

The Effects of Oxymatrine on the IL-10 Level in Lung Tissues of Asthmatic Rats

作　　者：龙兴云[1] 张平[1] 刘艳丰[1] 邓湘[1] 欧立文[2] 

机构地区：[1]南华大学附属第一医院呼吸内科,湖南衡阳421001 [2]湘南学院附属医院呼吸内科,湖南郴州423000

出　　处：《现代生物医学进展》2016年第16期3036-3039,共4页Progress in Modern Biomedicine

基　　金：湖南省教育厅科研基金项目(12A126)

摘　　要：目的:观察不同浓度氧化苦参碱(Oxymatrinem,Oxy)对哮喘大鼠肺组织IL^(-1)0表达的影响,并探讨其作用机制。方法:构建哮喘大鼠模型,将40只清洁级健康雌性SD大鼠随机分成5组,每组8只:A:哮喘组(仅卵蛋白(Ovalbumin,OVA)致敏)、B:低浓度组(Oxy 50 mg/kg)、C:中浓度组(Oxy 100 mg/kg)、D:高浓度组(Oxy 150 mg/kg)、E:对照组(生理盐水),末次激发24 h后处死全部大鼠,取大鼠肺脏,HE染色观察肺组织病理改变,采用RT-PCR、Western Blot测定各组肺组织中IL^(-1)0基因及蛋白水平的表达。结果:HE结果显示,哮喘组可见大量炎症细胞浸润,气管平滑肌明显增厚。对照组肺泡壁薄且光滑,未见明显炎性细胞的浸润,不同浓度氧化苦参碱药物干预组其肺组织炎症细胞浸润及气管平滑肌病变程度随着用药浓度的增高呈逐渐减轻趋势。RT-PCR以及Western blot检测IL^(-1)0发现,哮喘组、氧化苦参碱低浓度组、氧化苦参碱中浓度组与对照组相比IL^(-1)0的表达均有所减低(P<0.05),而氧化苦参碱高浓度组与对照组比较,IL^(-1)0的表达无统计学意义(P>0.05);氧化苦参碱中浓度组、氧化苦参碱高浓度组与哮喘组相比IL^(-1)0的表达均有所增高(P<0.05),氧化苦参碱低浓度组与哮喘组相比IL^(-1)0的表达无统计学意义(P>0.05)。结论:氧化苦参碱抑制、控制哮喘发作可能与促进肺组织中IL^(-1)0基因、蛋白的表达相关,且促进程度在一定范围内与浓度呈正比。Objective： To observe the influence of the different concentration of Oxymatrine on the level of IL-10 in lung tissues of asthmatic rats and explore its mechanism. Methods： 40 healthy female SD rats were randomly divided into five groups： A： the asthma model group（OVA）, B： low concentration group（Oxy 50 mg/kg）, C： medium concentration group（Oxy 100 mg/kg）, D： high concentration group （Oxy 150 mg/kg） and E： Control group （normal saline）. The rats were killed when they were nebulizated after 24 hours. The histological change of lung tissues was observed by HE staining. The expression of IL-10 in lung tissues was measured by RT-PCR and Western blot. Results： HE stainging demonstrated that a large number of inflammatory cells infiltration existed in lung tissues of asthmatic group, airway smooth muscle became think, excessive collagen deposition, but it did not obviously change in control group. inflammatory cells infiltration in lung tissues and airway smooth muscle change in different concentrations of oxymatrine drugs groups were gradually reduced. The expressions oflL-10 in asthma model group, low concentration group and medium concentration group was lower than that of control group （P 〈0.05）, but The expressions of IL-10 in high concentration group had no significant differences compared with control group （P 〉0.05）; The expressions of IL-10 in medium concentration group and high concentration group was higher than asthma model group （P 〈0.05）, but the expressions of IL-10 in low concentration group had no significant differences compared with asthma model group （P 〉0.05）. Conclusions： Oxymatrine can promote the expression of IL-10 in asthmatic rats, which was positively correlated in the concentration range of Oxy.

关 键 词：支气管哮喘 氧化苦参碱 IL-10 

分 类 号：R562.25[医药卫生—呼吸系统]