加味四物汤动员心肌梗死小鼠骨髓干细胞改善心室重构  

Modified Siwu decoction for mobilizing bone marrow stem cell to improve ventricular remodeling in mice myocardial infarction

作　　者：魏英[1,2] 王钰莹[1] 余丽梅[1] 方宁[1] 姚观平[1] 

机构地区：[1]遵义医学院附属医院贵州省细胞工程重点实验室,贵州遵义563003 [2]湖北医药学院附属东风医院实验中心,湖北十堰442000

出　　处：《重庆医学》2016年第15期2032-2035,共4页Chongqing medicine

基　　金：贵州省科技计划(黔科合S字[2007]1029);遵医(2006)034博士启动基金

摘　　要：目的观察加味四物汤(mSWT)对心肌梗死(MI)小鼠骨髓干细胞动员的影响,探讨mSWT改善心室重构的机制。方法结扎冠状动脉前降支建立小鼠MI模型,灌胃给予mSWT,小鼠被分为4组:假手术对照组,MI模型组,mSWT低剂量(5g·kg-1·d-1)组和mSWT高剂量(10g·kg-1·d-1)组,每组10只。行心肌组织病理学检查,流式细胞术分析造血干细胞(HSCs)和间充质干细胞(MSCs)占骨髓和外周血细胞的百分比。结果 mSWT高、低剂量组治疗14d后,较MI模型组小鼠左心室壁较厚,MI面积减小,梗死区域可见大量岛状分布的心肌细胞,心室重构明显改善。且小鼠骨髓和外周血中HSCs及MSCs细胞百分比明显增加(P<0.05)。结论 mSWT可能通过促进MI小鼠骨髓HSCs和MSCs的增殖和向外周动员,参与改善MI后的心室重构。Objective To observe the effects of modified Siwu decotion（mSWT）on bone marrow stem cell mobilization,and to investigate the mechanisms of mSWT for improving ventricular remodeling.Methods The mouse myocardial infarction（MI）model was set up by ligating the anterior descending coronary artery.Mice were intragastrically administrated with mSWT.The mice were randomly divided into 4groups：sham operation control group,MI model group,mSWT low dose（5g·kg-1·d-1）group and mSWT high dose（10g·kg-1·d-1）group,10 cases in each group.The myocardial tissue pathological examination was performed.The proportion of hematopoietic stem cells（HSCs）and mesenchymal stem cells（MSCs）to bone marrow and peripheral blood cells were analyzed by flow cytometry.Results After 14 dtreatment by 5,10g·kg-1·d-1 mSWT,the left ventricular wall was thickened compared with the model MI mice,the myocardial infarction area was decreased,large number of survival mycardial cells as island type distribution could be found in the infarction area,and the ventricular remodeling was significantly improved,moreover the percentages of HSCs and MSCs cells in bone marrow and peripheral blood were significantly increased（P〈0.05）.Conclusion mSWT might promote MI mouse bone marrow HSCs and MSCs proliferation and mobilization to periphery to participate in the ventricular modeling after improving the myocardial infarction.

关 键 词：心肌梗死 骨髓干细胞 加味四物汤 心室重构 

分 类 号：R285.5[医药卫生—中药学]