微悬臂生物传感器  

Microcantilever Biosensors

作　　者：戴莹萍 嵇正平[1] 王赪胤[1] 胡效亚[1] 汪国秀[2] 

机构地区：[1]扬州大学化学化工学院,扬州225002 [2]悉尼科技大学数学与物理科学学院,澳大利亚NSW2007

出　　处：《化学进展》2016年第5期697-710,共14页Progress in Chemistry

基　　金：国家自然科学基金项目(No.21375116);国家高技术研究发展计划(863)项目(No.2009AA03Z331);江苏省大型科学仪器设备共享服务平台分析测试技术及方法研究课题(BZ201409)资助~~

摘　　要：微悬臂生物传感器是基于原子力显微镜和生物传感器发展而来,具有无需标记、快速、实时、灵敏度高等优点,已广泛运用于生物医学、环境监测、医药、食品及军事等多个领域。微悬臂生物传感器已成为当前研究热点。本文对近年来微悬臂生物传感器的研究工作进行了综述,总结了微悬臂传感器中微悬臂梁的结构、工作模式、激励方法和检测方法。国内有关微悬臂传感器的综述多是着重于介绍微悬臂传感器的应用进展,而缺少对微悬臂传感器的检测方法或读出方式做全面而系统的介绍,本文不仅全面且系统地对微悬臂传感器的8种检测方法进行了详细的介绍和分析,还介绍了微悬臂梁的多种典型尺寸、外观和微悬臂传感器工作模式中的"热模式"。针对微悬臂传感器实现特定检测及提高灵敏度的目的,本文总结了微悬臂传感器的表面修饰方法,不仅介绍了国内外微悬臂生物传感器最新的应用动态,还介绍了一种具有自驱动自传感功能的新型微悬臂传感器,并对微悬臂生物传感器的应用前景作了探讨。Microcantilever biosensors based on atomic force microscope have the advantages of label-free,rapid,real-time,and high sensitivity detection features. Therefore,microcantilever biosensors can be applied in the fields of biomedicine,environmental monitoring,food production and military defenses. Microcantilever biosensors have become the focus of scientific research. In this paper,we reviewthe working principle,excitation method and detection mechanism of microcantilever biosensors. Previewreviews about the microcantilever biosensors mainly focuse on the progress of applications,but lack of comprehensive and systematic introduction on detection methods. Herein,we not only systematically analyze eight detection methods,but also introduce several typical sizes,appearance and ＂heat mode＂of microcantilever biosensors. So far,these have not been reported in literatures. In order to realize specific detection and improve sensitivity of microcantilever sensors,we summarize the surface modification methods of microcantilever sensors and reviewthe latest applications of microcantilever biosensors. Furthermore,we introduce a newkind of self-actuating and self-sensing microcantilever biosensors. The prospective of microcantilever biosensors is also discussed in this paper.

关 键 词：微悬臂 生物传感器 原子力显微镜 检测 应用 

分 类 号：O657.99[理学—分析化学]