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作 者:李红晓[1,2] 张殿朋[2] 卢彩鸽[2] 赵洪新[1,3]
机构地区:[1]沈阳师范大学化学与生命科学学院,辽宁沈阳100034 [2]北京市农林科学院植物保护环境保护研究所,北京100097 [3]浙江理工大学生命科学学院浙江省植物次生代谢重点实验室,浙江杭州310018
出 处:《微生物学杂志》2016年第2期87-92,共6页Journal of Microbiology
基 金:辽宁省科学公益基金项目(2014003020);北京市农林科学院青年科研基金(QNJJ201317);北京市优秀人才培养资助项目(2013D002020000005)
摘 要:解淀粉芽胞杆菌(Bacillus amyloliquefaciens)是与枯草芽胞杆菌相似,可产芽胞、革兰阳性(G+)、具有生防活性的益生细菌。其代谢过程中产生的抗菌物质种类多、抗菌谱广,其抗菌制剂或抗菌提取物具有无毒、无害、无残留、抑菌时效长等优点,可广泛应用于果蔬保鲜、果蔬生长期及采后微生物病害的生物防治,是极具开发价值的生防菌。本文从抗菌机理、抗性基因组成、基因工程菌构建、抗性产品开发及对微生物病害防治等几个方面,对解淀粉芽胞杆菌的研究及应用现状进行了归纳和总结,为其在农业生物工程中的规模化安全应用提供参考。Bacillus amyloliquefaciens is a strain similar to B.subtilis,a beneficial microbe that can produce spore,Gram-positive,and possessing biocontrolling activity.During its metabolism it produces antibiotic substance,a variety of antibiotic preparations or antibiotic extracts with broad antibiotic spectrum,non toxic,non harms,non residues,long effectiveness for a given period of time,and other advantages.It can be widely applied in keeping fruits and vegetables fresh,and in biocontrolling microbial pests during their growth and post-harvest.It is a very ideal biocontrolling microbe with the most developing value.In this paper the studies,the current application of B.amyloliquefaciens were drawn and summarized from aspect of the antibiotic mechanism,the antibiotic gene component,the establishment of genetic engineering strain,the development of resistance products,the controlling against microbial pests,and other aspects,and accordingly to provide references for its large-scale safety application in agricultural bio-engineering.
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