小鼠GCPⅡ基因剔除对脑外伤后脑水肿的影响  

GCPⅡgene knockout effect on brain edema after brain injury

作　　者：郭杨[1] 钟春龙[1] 包金岗 曹阳[1] 高阳[1] 徐巳奕[1] 郑彦[1] 罗其中[1] 江基尧[1] 

机构地区：[1]上海交通大学医学院附属仁济医院神经外科,上海200127

出　　处：《立体定向和功能性神经外科杂志》2016年第1期21-25,共5页Chinese Journal of Stereotactic and Functional Neurosurgery

摘　　要：目的研究普通小鼠和GCPⅡ基因剔除后小鼠脑外伤后脑水肿的变化,探讨并研究GCPⅡ基因剔除后对脑外伤后脑水肿的影响。方法雄性C57BL/6J小鼠随机20只,随机分为两组(C57BL/6J小鼠假手术组10只,C57BL/6J小鼠颅脑损伤组10只)。雄性GCPⅡ基因剔除小鼠随机20只,随机分为两组(GCPⅡ基因剔除小鼠假手术组10只,GCPⅡ基因剔除小鼠颅脑损伤组10只),应用PinPointTMPCI3000精细颅脑撞击仪,设定参数(打击深度为1.5mm;打击时间为80ms,打击的速度为1.5m/s),精确撞击小鼠脑皮质。伤后24h,行干湿重法测脑组织含水量,Western Blot检测AQP4蛋白的表达,MRI检测水肿程度,伊文思蓝检测血脑屏障破坏程度。结果GCPⅡ基因剔除小鼠较C57BL/6J小鼠外伤后神经功能明显改善(P<0.05);GCPⅡ基因剔除小鼠脑组织含水量、水肿体积以及外伤区域T2 WI信号强度较C57BL/6J小鼠降低(P<0.05)。结论小鼠的GCPⅡ基因剔除后脑外伤后脑水肿较C57BL/6J小鼠明显减轻,进一步证明小鼠GCPⅡ基因敲除后对脑外伤后脑组织的保护作用。Objective To study cerebral edema changes between C57BL/6Jmice and GCPⅡgene knockout mice and to investigate the relationship between GCPⅡgene knockout and cerebral edema after TBI.Methods A total of 20 male WT C57BL/6Jmice were randomly divided into two groups（WTC57BL/6J＋TBI group,n=10;WT C57BL/6J＋ sham TBI group,n=10）,20 male GCPⅡgene knockout mice were randomly divided into two groups（KO ＋ TBI group,n=10;KO ＋sham TBI group,n=10）.TBI model was made by CCI.Set the parameter（impact velocity 1.5m/s;reaching depth 1.5 mm;remained in the brain 80 ms）.The rotarod test,brain water content,edema volume and signal intensity ratio of T2 WI in the injury area were evaluated at 24 h after TBI.Results Our results showed that GCPⅡgene knockout mice administration improved neurological deficits after 24 hfollowing TBI（P0.05）.GCPⅡ gene knockout reduced brain water content,edema volume and signal intensity ratio of T2 WI in the injury brain area（P0.05）.Conclusion Our findings suggest that GCPⅡgene knockout exert its protective effect on brain by reducing cerebral edema after TBI.

关 键 词：颅脑损伤 GCPⅡ 基因剔除 脑水肿 磁共振成像 

分 类 号：R651[医药卫生—外科学]