脐血间充质干细胞移植联合紫杉醇对卵巢癌A2780细胞增殖、凋亡及侵袭的影响  

Effects of combined treatment using umbilical cord blood mesenchymal stem cell transplantation and paclitaxel on proliferation, apoptosis, and invasion of A2780 ovarian cancer cells

作　　者：陈逢振[1] 尹利荣[1] 

机构地区：[1]天津医科大学第二医院妇科,天津市120000

出　　处：《中国组织工程研究》2016年第20期2927-2932,2931+2929-2930+2932,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:深入进行卵巢癌肿瘤干细胞的研究,有助于以卵巢癌肿瘤干细胞为靶点,改进卵巢癌的治疗策略,提高卵巢癌的生存率。目的:探讨脐血间充质干细胞联合紫杉醇对卵巢癌A2780细胞增殖、凋亡、侵袭能力的影响。方法:将人卵巢癌A2780细胞分为3组:空白对照组(常规培养细胞)、脐血间充质干细胞组(加入1×10~6个脐血间充质干细胞悬液)、联合组(加入1×10~6个脐血间充质干细胞悬液的同时加入1μmol/L紫杉醇溶液250μL)。采用免疫荧光法检测卵巢癌A2780细胞CD133^+抗原表达,流式细胞仪、TUNEL染色法、Transwell小室侵袭实验检测卵巢癌A2780细胞的增殖、凋亡和侵袭能力。结果与结论:(1)空白对照组CD133^+阳性抗原表达量很多,明显高于脐血间充质干细胞组及联合组,差异有显著性意义(P<0.05);(2)联合组细胞增殖能力、侵袭能力明显减弱,凋亡细胞数明显增多,与其他两组比较差异有显著性意义(P<0.05);(3)结果表明,脐血间充质干细胞联合紫杉醇能抑制卵巢癌A2780细胞的增殖、侵袭,并促进其凋亡。BACKGROUND： An intensive study on ovarian cancer A2780 cells contributes to modify treatment strategies targeting ovarian cancer stem cells and enhance survival rate of ovarian cancer patients. OBJECTIVE： To investigate the effects of umbilical cord blood mesenchymal stem cells（UC-MSCs） transplantation combined with paclitaxel on proliferation, apoptosis, and invasion of A2780 human ovarian cancer cells in vitro. METHODS： A2780 human ovarian cancer cells were assigned into three groups： blank control group（routine cell culture）, UC-MSCs group（addition of suspension containing 1×10^6 UC-MSCs） and combined treatment group（combined addition of suspension containing 1×10^6 UC-MSCs and 250 μL of 1 μmol/L paclitaxel solution）. CD133~＋ antigen expression, proliferation, apoptosis, and invasion were determined by immunofluorescent staining, flow cytometry, TUNEL staining, and Transwell chamber invasion assay, respectively. RESULTS AND CONCLUSON： CD133~＋ antigen expression in the UC-MSCs group and combined treatment group was significantly decreased compared with the blank control group（P〈0.05）. Proliferation and invasion abilities were significantly decreased, while apoptosis cell number was significantly increased in the combined treatment group compared with the other two groups（P〈0.05）. These findings indicate that the combined treatment of UC-MSCs transplantation and paclitaxel can inhibit proliferation and invasion and promote apoptosis of A2780 human ovarian cancer cells.

关 键 词：胎血 间质干细胞 紫杉酚 卵巢肿瘤 组织构建 组织工程 脐血间充质干细胞 紫杉醇 卵巢癌 A2780细胞 细胞增殖 细胞凋亡 细胞侵袭 

分 类 号：R318[医药卫生—生物医学工程]