中药提取物白杨素以端粒为靶点抑制白血病细胞生长  

Chrysin, the extract of a traditional Chinese medicine inhibits the growth of leukemia cells by targeting telomere

作　　者：庄平[1,2] 胡源[3] 夏怡[2] 邹雅茹[1,2] 应亦林[1,2] 梁惠欣[1,2] 叶静[1,2] 陆一鸣[1,2] 

机构地区：[1]上海交通大学医学院附属瑞金医院中法生命科学和基因组研究中心,上海200025 [2]上海交通大学医学院附属瑞金医院急诊科,上海200025 [3]上海交通大学医学院附属瑞金医院耳鼻喉科,上海200025

出　　处：《内科理论与实践》2016年第1期42-46,共5页Journal of Internal Medicine Concepts & Practice

基　　金：国家自然科学基金项目(项目编号:81270433;81471400;81171846;81372099);上海市科学技术委员会科研计划项目(项目编号:13JC1404001)

摘　　要：目的 :探讨白杨素抑制白血病细胞株NB4的机制。方法 :通过免疫荧光染色技术结合端粒重复序列(CCCTAA)n的肽核酸(PNA)探针的原位杂交技术,观察端粒CCCTAA和DNA损伤蛋白53BP1的共定位,以测定端粒损伤的形成。结果:白杨素能抑制NB4细胞有效增殖的半数致死浓度为28μmol/L,而在端粒处CCCTAA的PNA探针的绿色荧光信号与53BP1抗体的红色荧光信号发生融合。白杨素处理过的NB4细胞早期凋亡(15.90±3.82)、晚期凋亡(14.41±3.87)远高于对照组(2.01±0.61、1.72±0.57)(P<0.05)。结论:白杨素有抑制细胞周期、诱导细胞凋亡、影响端粒保护染色体末端的能力;白杨素特异性增加端粒处的损伤可能是其阻滞细胞周期的机制。Objective To investigate the mechanism of inhibition on leukemia cells（NB4） by Chrysin. Methods By immunofluorescence staining technique and in situ hybridization of telomere repeat sequence（CCCTAA）n, the orientation of telomere CCCTAA and the DNA damage protein 53BP1 were assessed in order to determine the damage of telomere. Results The median lethal concentration of chrysin for NB4 cells was 28 μmol/L, and the green fluorescence signal of peptide nucleic acid（PNA） probe of CCCTAA at the telomere was fused with the red fluorescence signal of53BP1 antibody. The early apoptosis（15.90 ±3.82） and late apoptosis（14.41 ±3.87） in the treated NB4 cells were much higher than that in the control group[（2.01±0.61）,（1.72±0.57）, P〈0.05]. Conclusions Chrysin has the ability to inhibit cell cycle, induce cell apoptosis, and influence the protection of end of chromosome by telomere. Chrysin specifically increases the damage in telomere,which may be the mechanism to arrest the cell cycle.

关 键 词：白杨素 端粒 DNA损伤 白血病 

分 类 号：R285[医药卫生—中药学]