大豆疫霉MAPK基因的鉴定与转录分析  

Identification and transcriptional analysis of the MAPK genes in Phytophthora sojae

作　　者：叶文武[1] 李爱宁[1] 王晓莉[1] 王源超[1] 

机构地区：[1]南京农业大学植物保护学院,南京210095

出　　处：《植物病理学报》2016年第3期338-346,共9页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31401688);江苏省自然科学基金(BK20140698);国家杰出青年科学基金(31225022)

摘　　要：疫霉菌包括大豆疫霉等重要植物病原物,属于茸鞭生物界卵菌门,在进化上与真菌相差甚远;由于分离培养与遗传转化等相对困难,目前对其生长发育和致病机理的研究相对滞后。本研究综合运用基因组学和转录组学方法,首先对植物病原卵菌与真菌的蛋白激酶特别是MAPK进行了鉴定和比较,然后对大豆疫霉MAPK的基因结构、蛋白功能域以及转录模式等进行深入分析。结果表明,植物病原卵菌比真菌含有更多的蛋白激酶(包括MAPK),且卵菌及其近缘物种硅藻的MAPK与真菌在进化上相对独立;大豆疫霉的14个MAPK中,4个具有非典型的磷酸化唇序列,7个含有PH、C2、WW、PAS等与细胞信号转导相关的其它功能域;转录分析表明,大部分MAPK可能在大豆疫霉生长发育与致病的整个过程或特定过程中发挥重要作用。本文通过对蛋白激酶特别是MAPK的分析揭示了植物病原卵菌(与真菌相比)在细胞信号转导网络与机制上的独特性与复杂性,可为进一步研究疫霉菌MAPK的生物学功能及其信号调控机制提供参考。Phytophthora genus,including the significant causal agents of plant diseases such as Phytophthora sojae,belongs to the oomycetes in the kingdom of Stramenopiles,which are evolutionarily distant from ＇true＇fungi.Because gene knockout and multiple other conventional molecular genetic manipulation technologies are not feasible for Phytophthora species to date,researches on the molecular mechanisms of growth,development,and pathogenesis were hampered.In this study,we combined the integrated approaches of genomics and transcriptomics to identify and compare protein kinase genes,especially MAPKs,from several representative plant-pathogenic oomycetes and fungi,and then did further analyses on the MAPK genes in P.sojae,for example,gene structures,protein domains,and transcription patterns,etc..The results showed that the plant-pathogenic-oomycetes contain more protein kinases（including MAPKs） than fungi,and the MAPKs from oomycetes and relative species diatom exhibited a distant evolution relationship with fungi in phylogenetic study.A total of 14 MAPKs were found in P.sojae,in which 4 contained novel phosphorylation lip sequences in the MAPK domain,i.e.,SEY,THE,and TKH,and 7 contained additional functional domains related to intracellular signal transduction,i.e.,PH,C2,WW,and PAS.Transcription patterns indicated that most of the MAPKs played important roles in all or certain stages of growth,development,and pathogenesis of P.sojae.This study on protein kinases,especially MAPKs,revealed the specifity and complexity of oomycetes in intracellular signal transduction networks and mechanisms when compared with the fungi.These results will provide new insights for further study on biological functions and mechanisms of signal regulation of Phytophthora MAPKs.

关 键 词：疫霉菌 基因组 蛋白激酶 功能域 转录分析 

分 类 号：S432.1[农业科学—植物病理学]