滨蒿内酯对离体大鼠主动脉平滑肌作用的研究  

Vasodilator Effect of Scoparone on the Isolated Aorta of Rats

作　　者：李智[1] 刘洪瑞[1] 杨海玲[1] 丛华[1] 滕赞[1] 韩雪松[1] 

机构地区：[1]中国医科大学基础医学院药理学教研室,辽宁沈阳110001

出　　处：《中国医科大学学报》2002年第4期244-246,共3页Journal of China Medical University

基　　金：辽宁省科学技术发展基金资助项目 (982 2 60 0 5 )

摘　　要：目的 :研究滨蒿内酯 (scoparone ,Scop)对离体大鼠主动脉平滑肌张力的影响。方法 :通过测定离体血管环张力变化 ,观察Scop对主动脉平滑肌的舒张作用及与苯肾上腺素 (PE)、普萘洛尔 (Prop)和氯化钾 (KCl)之间的相互作用。结果 :Scop舒张离体主动脉平滑肌 ,并且对内皮完整 (EC +)标本的舒张作用明显大于去内皮 (EC - )标本 ,半抑制浓度 (IC50 )分别为 (10 0± 0 4 ) μmol/L(CE +)与 (83 2± 0 8) μmol/L(EC - ) (P <0 0 1) ;Scop使PE量效曲线下移 ,呈非竞争性抑制 ,亲和力指数 (PD2 )分别为 4 93± 0 0 5 (EC +)与 2 94± 0 14 (EC - ) ;Prop(1μmol/L)对Scop舒张血管平滑肌作用无影响 ;Scop不能抑制Prop所致血管平滑肌收缩 ;Scop对血管平滑肌的依内钙及依外钙性收缩均有明显抑制作用。结论 :Scop可舒张离体大鼠主动脉平滑肌 ,此作用与内皮细胞的功能有一定关系。﨩bjective: [WT5'BZ] This study was designed to investi ga te the possible mechanisms of the vascular dilatory effect of scoparone(Scop). [WT5'HZ]Methods: [WT5'BZ] Rings of rat thoracic aorta were studied in organ baths. We observed the vasodilator effect of Scop and its interactions with phen ylephrine(PE), propranolol(Prop), and potassium chloride(KCl). [WT5'HZ]Results: [WT5'BZ] Scop relaxed the isolated rat aortic smooth muscles precontracte d by 10 -6 mol/L PE,and IC 50 values were(10.0±0.4)μmol/L(EC+) and (83.2±0.8)μmol/L(EC-), respectively (P<0.01). Scop depressed the dose response cureves to PE in a dose dependent manner, with PD 2 value of 4.93 ±0.05(EC+) and 2.94±0.14(EC-), respectively. Vasodilator effect of Scop was no t affected by Prop(1 μmol/L). Scop did not depress the contractile responses to 60 mmol/L potassium chloride. Scop significantly inhibited the phasic and tonic contraction induced by PE(10 -6 mol/L) (P< 0.01 ). [WT5'HZ]Conclus ion: [WT5'BZ]Scop relaxed aortic smooth muscles, and the presence of endothel ium facilitated this vascular dilatory effect. Scop inhibited both the phasic an d tonic contraction, which may be the key mechanism of the vasodilator effect of Scop. [WT5'HZ]

关 键 词：离体大鼠 主动脉 滨蒿内酯 血管舒张 血管平滑肌 药理作用 

分 类 号：R285.5[医药卫生—中药学] R286.2[医药卫生—中医学]