局部缓释秋水仙素抑制移植血管内膜增殖的研究  

Inhibitory Effect of Local Delivery of Colchicine on Proli feration of Vascular Intima in Vein Graft

作　　者：杨巍[1] 罗涛[1] 刁彦鹏[1] 张强[1] 段志泉[1] 

机构地区：[1]中国医科大学第一临床学院外科

出　　处：《中国医科大学学报》2002年第4期252-254,共3页Journal of China Medical University

摘　　要：目的 :观察局部缓释秋水仙素 (colchicine ,COL)对移植血管内膜增生的抑制程度。方法 :采用大鼠自体静脉移植模型 ,外涂含不同浓度COL的Pluronic胶 ,于术后 14d处死取材 ,行HE ,Verhoeff弹力纤维染色、增殖细胞核抗原 (proliferatingcellularnuclearantigen ,PCNA)免疫组化染色 ,测量内膜厚度、内膜与中膜面积比、PCNA阳性细胞百分比 ,用流式细胞仪计数各期细胞百分比 ,观察不同浓度COL对内膜及平滑肌细胞增殖的抑制程度。结果 :血管移植术后 14d ,对照组的内膜厚度、内膜面积与中膜面积比 ,与高浓度组、中浓度组、低浓度组相比 ,差异显著(P <0 .0 5 )。PCNA阳性细胞数在各组之间相互比较 ,差异显著 (P <0 .0 5 )。流式细胞仪分析 ,从高浓度组、中浓度组、低浓度组到对照组 ,G0 G1、S期细胞所占比例逐渐减少 ,G2 M期细胞所占比例逐渐增多 (P <0 .0 5 )。结论 :局部缓释COL具有抑制移植血管内膜及平滑肌细胞增殖的作用。COL抑制血管内膜增殖作用与其剂量呈剂量—效应关系。COL可使平滑肌细胞停止在分裂中期 ,抑制其进入下一个增殖周期 ,从而抑制移植血管内膜及平滑肌细胞增殖的作用。﨩bjective: [WT5'BZ] The study was performed to investi ga te the inhibition of the proliferation of vascular intima and smooth muscle cell (SMC) by colchicine (COL ) of high local concentrations at the site of vein gra ft and to investigate the cause of vein graft stenosis. [WT5'HZ]Methods: [W T5'BZ] An animal model of autogenous vein graft through transplanting the jugul ar vein into abdominal aorta was established in 48 rats.The rats after operation were randomly divided into 4 groups.GroupⅠwas control group (only pluronic F 127 gel without any COL). GroupⅡreceived low concentration of COL in F 127 gel ( 0.044 mg/ml). Group Ⅲ received middle concentration of COL in F 127 gel (0.22mg /ml) . Group Ⅳ received high concentration of COL in F 127 gel (0.44 mg/ml). The vein grafts were collected 14 days after operation. The ne ointimal thickness and ratio of intimal area to medial area were measured by us ing computer graph analysis system. The positive cells of proliferating cellular nuclear antigen (PCNA) were detected to indicate the degree and the cause of hy perplasia of intima and SMC. Cell suspension was fixed with alcohol(70%),dyed w ith propidium iodide (PI),and detected with flow cytometry. Flow cytometry coun ted 10 000 cells,and computed results by DNA content and ratio in total cells of each cell cycle. [WT5'HZ]Results: [WT5'BZ] The neointimal thickness were sig nificantly reduced in groupⅡ, group Ⅲ, and groupⅣ compared with groupⅠ(P <0.05). The ratios of intimal area to medial area significantly decreased in groupⅡ, group Ⅲ, and group Ⅳ compared with groupⅠ(P<0.05).The positiv e cells of PCNA in groupⅡ, group Ⅲ, and group Ⅳ were less than that in group Ⅰ 14 days after operation(P<0.05). The cell cycle distribution were sig nificantly different between the groups. [WT5'HZ]Conclusion: [WT5'BZ]Local delivery of COL has the effect on inhibition of proliferation of intima and SMC of vein graft. The COL can stop the SMC

关 键 词：局部缓释 秋水仙素 移植血管 内膜增殖 流式细胞仪 动物模型 血管狭窄 

分 类 号：R622.4[医药卫生—整形外科]