大鼠烫伤早期骨骼肌代谢高通量芯片中长链非编码RNA的变化及意义  

Expression and significance of long noncoding RNA in tibialis anterior muscle tissues of burned rats at early stage

作　　者：张海军[1] 郝岱峰[1] 柴家科[1] 郁永辉[1] 

机构地区：[1]解放军总医院第一附属医院烧伤整形科,北京100048

出　　处：《解放军医学院学报》2016年第7期766-768,783,共4页Academic Journal of Chinese PLA Medical School

基　　金：国家自然科学基金项目(81120108041;81471873;81201466);总后勤部全军"十二五"重点科技项目(BWS14J048;BWS14J049)~~

摘　　要：目的探索大鼠严重烫伤后早期骨骼肌代谢相关长链非编码RNA(long noncoding RNA,lnc RNA)的变化及其意义。方法建立严重烫伤大鼠模型,将实验组大鼠背部置于94℃热水13 s,造成大鼠30%体表面积Ⅲ度烫伤;对照组大鼠置入37℃温水13 s,其他同实验组。于伤后72 h留取胫骨前肌标本;应用总RNA提取试剂盒抽提胫骨前肌总RNA,采用Arraystar公司Rat Lnc RNA Array(4×44 K,Arraystar),分别检测实验组与对照组胫骨前肌标本中MRBC025014和MRAK080917两种长链非编码RNA的表达水平,实时定量PCR验证以检验芯片数据的可靠性。结果芯片数据结果显示,实验组MRAK080917、MRBC025014的表达水平较对照组显著降低(P=0.028,P=0.018),进一步的PCR验证结果证实,实验组较对照组MRAK080917和MRBC025014相对表达水平均降低(P<0.05)。结论大鼠严重烫伤后早期骨骼肌组织中MRBC025014和MRAK080917表达水平明显降低,其参与骨骼肌代谢的机制可能与其调控靶基因相关。Objective To explore the significance and changes of long noncoding RNA in metabolism of skeletal muscles at early stage in burned rats.Methods The rats were divided into experimental group（T） and control group（C）.Within 72 h after the injury,the tibialis anterior（TA） muscles were collected;TRIZOL method was applied for extraction of total RNA in tibialis anterior muscle,Arraystar company Rat Lnc RNA Array（4×44 k Arraystar） was used to detect the expression level of MRBC025014 and MRAK080917 in the tibialis anterior muscle of both experimental group and control group,real time quantitative PCR was used to verify the reliability of the microarray data.Results The chip screening results showed that compared with control group,the expression levels of MRAK080917 and MRBC025014 were significantly lower in experimental group（P=0.02,P=0.018）.PCR data confirmed that the expression levels of MRAK080917 and MRBC025014 in experimental group were consistent with the results of chip screening（P〈0.05）.Conclusion The expression levels of MRBC025014 and MRAK080917 down-regulate significantly in skeletal muscle at early stage after severe burn of rats,and its function in skeletal muscle metabolism may be related to regulating target genes.

关 键 词：长链非编码RNA 烫伤 大鼠 骨骼肌 

分 类 号：R644[医药卫生—外科学]