5株鼠样品中分离汉坦病毒毒株S基因序列比较与分析  

Sequence comparison and analysis of S Gene of five Hantavirus strains separated from rodents

作　　者：胡群[1] 邹春颖[1] 马思杰[1] 童淑梅[1] 梅勇[1] 

机构地区：[1]大榭出入境检验检疫局,宁波315812

出　　处：《中国人兽共患病学报》2016年第5期502-505,共4页Chinese Journal of Zoonoses

摘　　要：目的了解近年来从宁波口岸捕获的鼠类样品中分离的5个汉坦病毒株遗传学差异。方法利用RT-PCR方法,对5个汉坦病毒株S基因全长进行扩增、克隆和测序,并将这5个毒株的S基因序列分别与GenBank登录的20个汉坦病毒感毒株进行比较分析。结果 DX1507株和DX1408株S基因核苷酸序列长度为1766bp与汉城型(SEOV)病毒株RnDH27同源性最为接近,LJ1112株S基因核苷酸序列长度为1772bp与汉城型(SEOV)病毒株Cherwell同源性最为接近,DX0903株和BL1402株S基因核苷酸序列长度为1725bp与大别山型(DABV)病毒株Yongjia-Nc-95和Yongjia-Nc-38同源性最为接近。结论宁波口岸分离到的汉坦病毒株分别为汉城型和大别山型,对宁波口岸开展汉坦病毒传播防控有重要意义。To know the genetic differences of five hantavirus strains separated from rodents captured in Ningbo port area.The S genes of five hantavirus strains were amplified,cloned and sequenced.Then the S gene sequences of these strains were compared with that of 20 hantavirus strains in GenBank.The results showed that the DX1507 and DX1408strains S segment are 1766 bp in length and with closest homology to the SEOV virus strain Rn-DH27,the LJ1112 strains S segment are 1772 bp in length and with closest homology to the SEOV virus strain Cherwell,the DX0903 and BL1402strains S segment are 1725 bp in length and with closest homology to the DOBV virus strain Yongjia-Nc-95 and Yongjia-Nc-38.This study indicated the five hantavirus strains separated from rodents captured in Ningbo port area are belong to SEO or DOBV type,which has an important significant to control hantavirus spreading in Ningbo port.

关 键 词：汉坦病毒 宁波 序列分析 S基因 

分 类 号：R373[医药卫生—病原生物学]