RP-HPLC法同时检测短梗五加果中4个有效成分的含量  

Simultaneous determination of four effective compounds of Acanthopanax sessiliflorus fruits by RP-HPLC

作　　者：孟永海[1] 王欣慰[1] 吴高松[1] 翟春梅[1] 杨春娟[1] 宋洋[1] 王知斌[1] 

机构地区：[1]教育部北药基础与应用研究重点实验室黑龙江省中药及天然药物药效物质基础研究重点实验室黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《化学工程师》2016年第5期27-29,共3页Chemical Engineer

基　　金：黑龙江省青年科学基金项目(QC2012C064);国家自然科学基金青年科学基金项目(81303195);国家重点基础研究发展计划(973计划)2013CB531801

摘　　要：目的:建立反相高效液相色谱-紫外法测定短梗五加果中4个有效成分含量。方法:采用Diamasil C_(18)色谱柱(200×4.6mm,5μm),以乙腈-0.05%磷酸为流动相,流速为1mL·min^(-1),检测波长为210nm,柱温为25℃。结果:短梗五加果中4个有效成分的分离度良好,标准曲线在检测范围内均呈良好线性,r=0.9997~0.9999,平均回收率95.6%~101.6%,RSD为2.4%~3.3%。结论:该方法简便可靠,精密度高,重复性好,适用于短梗五加果有效成分含量测定方法。Objective： To establish a RP-HPLC-UV method for the determination of four effective compounds of Aeanthopanax sessiliflorus. Methods： Chromatographic conditions were gave on an C18 column （200×4.6mm, 5μm）at 25℃, an isocratic mobile phase consisting of acetonitrile-0.05% phosphoric acid solution at a flow rate of 1mL·min-1 and 210 nm detection wavelength. Results： four effective compounds of Acanthopanax sessiliflorus had good separating and linearity. The linear range of four effective compounds was wide（r=0.9997-0.9999）, the aver- age recovery was 95.6-101.6% and RSD was 2.4-3.3%. Conclusion： The method is simple and reliable, high precision, good reproducibility, and accurate for the quantitative analysis of four effective compounds of Acanthopanax sessiliflorus.

关 键 词：短梗五加果 有效成分 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]