拟南芥sscd1–1点突变对其表达的影响  

Effects of Arabidopsis point mutation sscd1-1 on its expression

作　　者：黄弈[1] 韩成云[3] 支添添[1] 任春梅[1,2] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]作物基因工程湖南省重点实验室,湖南长沙410128 [3]宜春学院化学与生物工程学院,江西宜春336000

出　　处：《湖南农业大学学报（自然科学版）》2016年第3期247-250,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目（30671121）;江西省科学技术厅青年科学基金计划项目（20151BAB214011）

摘　　要：为了探明拟南芥sscd1–1突变体中sscd1–1突变基因表达水平的降低是由剪切效率所致还是由自身启动子影响所致,分别构建了由外源35S启动子驱动的SSCD1正常基因和sscd1–1突变基因的表达载体及由内源自身启动子驱动的SSCD1正常基因和sscd1–1突变基因的表达载体,并将其转入到拟南芥sscd1–1突变体中。采用RT–PCR分析sscd1–1突变基因的表达。结果显示：外源35S启动子驱动的sscd1–1突变基因的表达与正常基因相比没有明显差别,而内源自身启动子驱动的sscd1–1突变基因的表达显著降低,这表明sscd1–1的点突变没有影响其剪切效率;sscd1–1突变基因表达水平的降低是由内源自身启动子影响所致。To investigate whether the reduction of the gene＇s transcription level from mutated sscd1-1 in Arabidopsis is induced by splicing efficiency or its own promoter regulation, expression vectors of exogenous 35S promoter and endogenous promoter driven by normal SSCD1 gene and sscd1-1 mutated gene respectively were firstly constructed, then it was transformed into Arabidopsis sscd1-1 mutant, the expression of the sscd1-1 mutated gene was analyzed by RT-PCR. The results showed that its expression did not affected by 35S promoter driven by sscd1-1 mutated gene, however, the expression level of sscd1-1 mutated gene driven by its endogenous promoter was significantly decreased. Hence, the point mutation in sscd1-1 did not affect the splicing efficiency of the sscd1-1 gene, the reduction of sscd1-1 expression level was caused by its endogenous promoter.

关 键 词：拟南芥 sscd1–1 启动子 基因表达 

分 类 号：Q786[生物学—分子生物学]