甘蓝型油菜BnaSOT12a基因的抗性作用研究  

Study on the resistance of BnaSOT12a gene in Brassica napus

作　　者：欧阳宁[1] 金璐[1] 阮颖[1] 陈东红[1] 

机构地区：[1]湖南农业大学生物科学技术学院

出　　处：《湖南农业大学学报（自然科学版）》2016年第3期251-255,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省高校创新平台开放基金项目（14K046）

摘　　要：磺酸转移酶（SOT,EC2.8.2.–）催化的磺酸化反应在植物的生长发育和植物抗逆过程中起着重要的作用。甘蓝型油菜BnaSOT12a基因是与拟南芥中AtSOT12基因同源的一个磺酸转移酶基因。为探寻BnaSOT12a基因的抗性相关功能,对其在油菜中不同部位的表达和在拟南芥中过表达情况下对转基因植株的影响进行了分析。结果显示：BnaSOT12a基因在甘蓝型油菜的根、叶、花芽和花中都有表达,其在花中的表达量最高,而在茎与果荚中没有表达;甘蓝型油菜BnaSOT12a基因在拟南芥中的过表达,能提高转基因植株在发芽和根生长方面对NaCl胁迫的耐受能力,但在盐胁迫下,BnaSOT12a的磺酸化作用并不影响拟南芥中抗病途径的信号传导,与抗病途径无关。Sulphonation reaction catalyzed by sulfotransferase （SOT, EC 2.8.2.-） plays a key role in plant growth and resistance. BnaSOT12a in Brassica nupus is a sulfotransferase gene, homologous to ATSOT12 in Arabidopsis. To seek BnaSOT12a gene functions associated with resistance, the gene expression level in various organs ofBrassica nupus and the effect of overexpression on transgenic plant of Arabidopsis were analyzed. Results showed that BnaSOT12a could be expressed in leaves, buds and flowers. In flowers, the expression lever was the highest. However, no expression in stems and capsule. The endurable capability of NaC1 was enhanced in root growth and germination in transgenic plant as BnaSOT12a overexpression in Arabidopsis. Nevertheless, disease-resistant signal transduction within Arabidopsis disease resistance pathway. sulphonation reaction catalyzed by BnaSOT12a did not affect under salt stress. Hence, BnaSOT12a seems irrelevant with

关 键 词：甘蓝型油菜 拟南芥 BnaSOT12a基因 半定量PCR 植物抗盐 基因表达 

分 类 号：Q78[生物学—分子生物学]