巨噬细胞系及PLEKHQ1基因敲除小鼠实验固定夹设计  

Design and development of a fixed holding apparatus for mice of macrophage system and PLEKHQ1 gene knockout

作　　者：刘宏举[1] 张鹏飞[1] 张硌[1] 

机构地区：[1]解放军第307医院医学工程科,北京100071

出　　处：《中国医学装备》2016年第5期124-126,共3页China Medical Equipment

基　　金：国家自然科学基金(31400739)"PH结构域蛋白PLEKHQ1协调巨噬细胞迁移与激活的机制研究"

摘　　要：目的:设计研制巨噬细胞系及PLEKHQ1基因敲除小鼠实验固定夹,为小鼠尾部采血和注射实验提供安全、简易、方便及省时的实验工具。方法:采用50 ml注射器按图纸开槽打孔,制作成巨噬细胞系及PLEKHQ1基因敲除小鼠实验固定夹;使用50 ml塑料试管及合适尺寸的塑料底托,制作成小鼠实验固定夹底座。结果:该小鼠固定夹与目前市场销售的小鼠固定夹相比较,具有制作简单、成本低廉、使用安全及操作简便省时等优点。结论:该小鼠实验固定夹为小鼠进行尾部静脉采血及注射等实验操作提供了安全、便利的实验工具,极大的提高了实验的工作效率。Objective：To develop a fixed clamp apparatus for Macrophage system and PLEKHQ1 gene knockout mice, in order to provide a safe, simple, convenient and time-saving experimental tool for mice tail blood collection and injection.Methods：Clamp for macrophage system and PLEKHQ1 gene knockout mice was made by 50 ml syringe with slot according to the drawing. Clamp base was made by 50 ml plastic tube and appropriate size of the plastic tap.Results：Compared with current mice clamp in the market, the mice fixed clamp was a simple, low-cost, safe, easy and timesaving tool.Conclusion： The mice clamp for mice tail blood collection and injection provided a safe and convenient tool, and greatly improved the efficiency of the experiment.

关 键 词：巨噬细胞系及PLEKHQ1基因敲除小鼠 实验 固定夹 设计 

分 类 号：R197.39[医药卫生—卫生事业管理]