大豆GmGLP10基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of GmGLP10 in Soybean

作　　者：张宇航[1] 李永光[1] 王雪松[1] 孙铭阳[1] 勾涵 陈薇[1] 李悦[1] 李文滨[1] 

机构地区：[1]东北农业大学农学院大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室,黑龙江哈尔滨150030

出　　处：《大豆科学》2016年第3期388-393,共6页Soybean Science

基　　金：抗逆转基因大豆新品种培育(2015ZX08004002);东北农业大学"青年才俊"项目(14QC26)

摘　　要：通过PCR的方法从大豆抗菌核病品种Maple Arrow中克隆得到Gm GLP10基因,生物信息学分析结果显示Gm GLP10蛋白由213个氨基酸组成,具有一个糖基化位点和多个磷酸化位点,为胞外分泌蛋白。通过对Gm GLP10基因起始密码子上游1 500 bp序列进行顺式作用元件分析,预测Gm GLP10启动子上具有多个与激素和防御胁迫应答相关的顺式作用元件。进化树分析结果表明,Gm GLP10与多个生长素结合蛋白进化距离较近,推断其可能具有相似的功能。Gm GLP10基因在菌核病菌胁迫下的转录本丰度的变化结果表明在菌核胁迫处理后Gm GLP10基因表达量上调明显。Gm GLP10可能作为生长素结合蛋白参与调控大豆的生长发育与抗病防御应答反应。GmGLP10 gene was isolated from soybean Sclerotinia Sclerotiorum resistant cultivar Maple Arrow by PCR method.Bioinformaties analysis showed that GmGLP10 gene encoding a 213 amino acids protein,with a N-glycosylation site and several phosphorylation sites and was putatively to be a extracellular protein. The Upstream 1 500 bp sequence of GmGLP10 gene were analyzed,multiple cis-elements related to hormone and defense stress responses existed in the GmGLP10 promoter region. Phylogenetic analysis results indicted that GmGLP10 and several Auxin binding proteins（ ABPs） had closer genetic relationship,that may have similar functions. The GmGLP10 transcript abundance was significantly increased after Sclerotia infection. These results suggested GmGLP10 may function as an Auxin binding protein to regulate growth and development of soybean and disease resistance defense responses.

关 键 词：大豆 GmGLP10基因 克隆 菌核病 生长素结合蛋白 

分 类 号：S565.1[农业科学—作物学]