大豆异黄酮和皂甙对H_(22)小鼠肝癌移植瘤的生长抑制及促细胞凋亡作用  

Anti-tumor and Pro-apoptotic Effects of Soy Isoflavones and Saponins on H_(22)-bearing Mice

作　　者：郑峰[1,2] 金延华[1,2] 何鑫[1,2] 全吉淑[1,2] 尹学哲[1,2] 

机构地区：[1]延边大学附属医院,吉林延吉133000 [2]延边大学医学院,吉林延吉133000

出　　处：《大豆科学》2016年第3期494-497,共4页Soybean Science

基　　金：国家自然科学基金(81160539;81360651)

摘　　要：为了探讨大豆异黄酮和皂甙对H_(22)小鼠肝癌移植瘤的生长抑制及促细胞凋亡作用,建立小鼠皮下H_(22)移植瘤模型,将其分为模型组、5-氟尿嘧啶组、大豆异黄酮组和大豆皂甙组。大豆异黄酮和大豆皂甙组分别按200 mg·kg^(-1)剂量每日灌胃给药,共10次;5-Fu组按25 mg·kg^(-1)剂量隔日腹腔注射给药,共5次。实验末期处死动物,计算抑瘤率、胸腺指数和脾脏指数,苏木素-伊红(HE)染色法观察肿瘤组织病理学变化,比色法检测肿瘤组织Caspase-3和Caspase-8相对活性以及血清还原型谷胱甘肽(GSH)含量、总抗氧化活力(T-AOC)和丙二醛(MDA)含量。结果表明:与模型组比较,大豆异黄酮和皂甙处理均能显著降低H_(22)小鼠肝癌移植瘤组织的瘤重,提高抑瘤率,其抑癌率分别为36.3%和34.8%。同时,大豆异黄酮和皂甙均显著升高荷瘤小鼠脾脏指数,增高肿瘤组织Caspase-3和Caspase-8相对活性,降低小鼠血清MDA水平,增高血清GSH和T-AOC水平。试验说明大豆异黄酮和皂甙对H_(22)小鼠肝癌移植瘤具有明显的抑瘤作用,其作用可能与其促细胞凋亡和抗氧化作用有关。The anti-tumor and pro-apoptotic effects of soy isoflavones and saponins on H22-bearing mice were investigated.Mice bearing H22 hepatoma cells were randomly assigned into 4 groups including model,5-Fu,isoflavone and soyasaponin groups. Animals were treated with ig 200 mg·kg^（-1）of soy isoflavones and saponins once daily for 10 days,and with ig 5-Fu 25mg·kg^（-1）every other day for 3 times. At the end of experiment,the tumor growth inhibition ratios,spleen and thymus indexes were calculated,the pathological changes were detected with hematoxylin eosin（ HE） staining,and the relative activities of Caspase-3 and Caspase-8,as well as the serum levels of reduced glutathione（ GSH）,the total antioxidant capacity（ T-AOC）and malondialdehyde（ MDA） were detected by the colorimetric method. The results showed that the administration with soy isoflavones and saponins inhibited the growth of transplanted tumor with the inhibitory ratios of 36. 3% and 34. 8%,respectively. In addition,soy isoflavones and saponin treatment elevated the spleen index,increased the relative activities of Caspase-3 and Caspase-8 of tumor tissue,decreased the serum MDA,and increased the serum GSH and T-AOC of H22-bearing mice. It is suggested that soy isoflavones and saponins could exhibit the inhibitory effects on the transplanted H22 tumor in mice,probably via pro-apoptotic and anti-oxidative activities.

关 键 词：大豆异黄酮 皂甙 H22 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]