艾纳香分株苗左旋龙脑稳定性及其优良单株筛选  

Stability Analysis of L-borneol and Selection of Optimum Individual Plant in the Divided Plants of Blumea balsamifera

作　　者：陈策[1,2,3] 于福来[2,3] 庞玉新[2,3] 杨全[1] 陈振夏[2,3] 黄梅[1,2,3] 刘立伟[1,2,3] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [3]海南省艾纳香工程技术研究中心,海南儋州571737

出　　处：《贵州农业科学》2016年第5期124-126,共3页Guizhou Agricultural Sciences

基　　金：国家自然科学基金"艾纳香叶片形态与左旋龙脑关联及其遗传连锁机制研究"(81303171);国家自然科学基金"黎药‘娜龙’品种整理及其用药规律研究"(81374065);海南省自然科学基金"艾纳香化学成分时空变异规律研究"(814350)

摘　　要：为探明艾纳香母株与分株苗间左旋龙脑含量的稳定性,同时筛选优良单株,选择10个分株苗无性系分别在3个不同地的土壤(澄迈、儋州和临高)进行盆栽试验,采用GC法测定左旋龙脑含量,应用相关分析和方差分析等对母株和分株苗间差异进行统计。结果表明:艾纳香母株和分株苗间左旋龙脑含量呈极显著相关(P<0.01);分株苗左旋龙脑含量受种质影响显著,其方差贡献率达69.65%,且在3种土壤下的广义遗传力(重复力)均达0.8以上;分株苗C33-3、C5-1和C5-4的左旋龙脑含量较高,均大于14mg/g,可作为较优材料进行生产扩繁。To study the contents’ stability of l-borneol and select the optimum individual plant,which will offer the materials and scientific basis for variety selection and division propagation of B.balsamifera.The divided plants of B.balsamifera from 10 clones were growing in three different kinds of soil（Chengmai,Danzhou and Lingao）and the contents of l-borneol was determined by gas chromatography and the raw data were analyzed by the ways of simple correlation analysis combined with variance analysis.Results：The correlation about the contents of l-borneol between maternal plants and divided plants was very significantly positive（P〈0.01）;The contents of l-borneol were influenced apparently by the genes,whose variance proportion was 69.65% and broad-sense heritability in three different kinds of soil were higher than 0.8;Tree excellent plants were selected on the basis of the contents of l-borneol（over14mg/g）and used for propagation,whose names were called C33-3、C5-1and C5-4,respectively.

关 键 词：艾纳香 分株苗 左旋龙脑 遗传稳定性 单株选择 

分 类 号：S567[农业科学—中草药栽培]