鲁禽3号麻鸡优良肉质性状分子遗传标记筛选  

Screening of Molecular Genetic Markers Applied to Excellent Meat Traits of Luqin No.3 Chicken

作　　者：王丹丹[1] 周艳[2] 雷秋霞[2] 韩海霞[2] 林浴霜[1] 

机构地区：[1]山东大学生命科学学院,山东省动物细胞与发育生物学重点实验室,山东济南250100 [2]山东省农业科学院畜牧兽医研究所,山东省畜禽疫病防治与繁育重点实验室,山东济南250100

出　　处：《中国家禽》2016年第9期10-14,共5页China Poultry

基　　金：山东省科技发展计划(2014GNC110028);山东省自然科学基金重点项目(ZR2014CZ003);山东省自然科学基金三院联合项目(ZR2014YL024)

摘　　要：脂肪酸结合蛋白(FABP)参与细胞内脂肪代谢,在鲁禽3号麻鸡体内不同组织中特异性表达7种FABP基因。试验旨在从7种FABPs中寻找到能够应用于家禽优良肉质性状筛选及育种的分子遗传标记。提取鲁禽3号麻鸡血细胞基因组DNA,分别扩增出FABP1-7基因全部外显子,采用测序与酶切结合的方法检测SNP位点的位置,并进行基因型与生产性状的关联性分析。结果表明:7种FABP基因中存在9个SNP位点,其中在鲁禽3号麻鸡体内能够用作优良肉质筛选与育种的分子遗传标记是FABP1-E2-83(外显子2第83位)、FABP4-E1-51(外显子1第51位)两个SNP位点。Fatty acid binding protein （FABP）participates in fat metabolism in cells. Seven FABPs genes were found expressed in Luqin No.3 chicken （LQ 3 chicken）. The experiment was aimed to find the appropriate SNP sites used as molecular genetic markers for breed of poultry with excellent meat traits. LQ 3 chicken genomic DNA were extracted from blood cells, and all exons of seven FABPs were amplified. The position of SNPs loci was tested by se- quencing or enzyme digestion. Then the correlation analysis of genotype and meat traits was made. According to the resuits,nine SNPs were found in 7 FABP genes,and only FABP1-E2-83 and FABP4-E1-51 could be used as molecu- lar genetic markers in screening and breeding of LQ 3 chicken with excellent meat traits.

关 键 词：FABP基因 单核苷酸多态性 分子遗传标记 肉质性状 

分 类 号：S831.2[农业科学—畜牧学]