复合因素致肠源性内毒素血症大鼠肝功能变化的研究  

Study on the Liver Function of Rats with Intestinal Endotoxemia Induced by Composite Factor

作　　者：赵国荣[1] 毛娅男[1] 李灿[1] 何宜荣[1] 李雄安 连俊凯 陈研焰 

机构地区：[1]湖南中医药大学中医学院,湖南长沙410208

出　　处：《中医药导报》2016年第12期25-26,34,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省教育厅重点项目(12A101);湖南中医药大学中医临床基础重点学科资助项目;湖南省方剂学重点学科资助项目

摘　　要：目的:探讨复合因素致肠源性内毒素血症(IETM)大鼠肝功能的变化。方法:将20只SD大鼠随机分为正常组和模型组,每组10只。用高脂、糖喂养模型组大鼠,同时予白酒(52°北京红星二锅头)灌胃,正常组予普通饲料且灌胃等剂量蒸馏水。12周后观察大鼠饲养情况,检测大鼠内毒素(LPS)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、谷氨酰转肽酶(GGT)指标及肝组织病变。结果:与正常组对比,模型组大鼠嗜睡、食欲不佳、毛发枯萎、大便溏;模型组血浆LPS升高(P<0.05),血清ALT、AST、ALP、GGT升高(P<0.05);模型组大鼠肝组织可见坏死、脂肪空泡及炎性浸润,正常组肝小叶轮廓清晰,未见脂肪变性、炎性改变。结论:复合因素可致机体肝损伤,其作用机制与IETM的形成有关,中医病因学说中高脂、糖、酒饮食致病与西医学的饮食致IETM肝损伤呈正相关。Objective： To investigate the changes of the liver function of rats with intestinal endotoxemia （IETM） induced by composite factors. Methods： The 20 SD rats were randomly divided into normal group and model group, and 10 rats in each group. The model group was fed up with high amounts of sugars, fats and intragastic administration of 52% Beijing Red Star Erguoto liquor. Meanwhile the normal group was given ordinary food and administered with an equal volume of normal saline. After 12 weeks, plasma levels of lipopolysaccharide （LPS）, serum levels of alanine transaminase （ALT）, aspartate transaminase （AST）,^-glutamyl transpeptiolase （GGT）, alkaline phosphatasa （ALP） and pathological changes of hepatic tissues were determined. Results： Compared with the normal group, the symptoms of the rats in model group were drowsiness, poor diet, hair withered and loose stool. The plasma LPS of the model group increased （P〈0.05）, and the serum ALT, AST, ALP, GGT alao increased （P〈0.05）. In the model group, there were necrosis, fat vacuoles and inflammatory infiltration in liver tissue, while in the normal group, the outline of the hepatic lobe was clear, and there was no fatty degeneration and inflammatory changes. Conclusions： Composite factors can lead to liver injury, and its mechanism of action is related to the formation of IETM. The diet pathogenesis theory of high fat, sugar, wine in TCM was positively correlated with diet causes IETM liver damage in modern medicine.

关 键 词：复合因素 肠源性内毒素血症 肝损伤 中医病因学 

分 类 号：R285.5[医药卫生—中药学]