HPLC法同时测定金黄凝胶中5种成分的含量  

Simultaneous Determination of Five Components in Jinhuang Gel(金黄凝胶) by HPLC

作　　者：顾小燕[1] 朱全刚[1] 张若曦[1] 袁松涛[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院药剂科,上海200437

出　　处：《中医药导报》2016年第12期51-53,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海中医药大学预算内项目(2013JW51);上海市科委中医重点项目(No.13401902002);上海市卫计委进一步加快中医药事业发展三年行动计划(2014年-2016年)项目(ZY3-JSFC-2-3006)

摘　　要：目的:通过梯度洗脱的方法,建立了金黄凝胶中芦荟大黄素、大黄酸、姜黄素、大黄素、大黄酚的含量测定方法。方法:采用ZORBAX SB-C18色谱柱(4.6×250 mm,5μm),以乙腈-4%冰醋酸为流动相梯度洗脱,柱温:30℃,检测波长:430 nm,流速:1.0 m L·min^(-1)。结果:在此条件下5种成分能够得到很好的分离,芦荟大黄素、大黄酸、姜黄素、大黄素、大黄酚的线性范围分别为0.316~6.32μg·mL^(-1)(r=1.0000,n=5),0.536~10.72μg·mL^(-1)(r=0.9999,n=5),0.325~6.50μg·mL^(-1)(r=1.0000,n=5),0.57~11.40μg·mL^(-1)(r=1.0000,n=5),0.491~9.82μg·mL^(-1)(r=1.0000,n=5)。平均加样回收率(n=6)分别为99.7%,98.8%,99.2%,97.4%,99.9%,RSD%分别为2.47%,2.55%,2.64%,1.50%,2.54%。结论:该方法简便、快速、准确,重复性好,可用于控制金黄凝胶的质量。Objective： To establish a HPLC method for quantitative determination of aloe / emodin, rhein, curcumin,emodin and chrysophanol in Jinhuang gel （金黄凝胶） using gradient elution method. Methods： HPLC method was used, with ZORBAX SB-C18 column, acetonitrile-4% acetic acid, linear gradient elution, column temperature was maintained at 30℃, as mobile phase with a flow rate of 1.0 mL·min-1, and detecting wave-length 430 nm. Results： In the chromatographic condition, the five components were completely separated. The calibration curves of aloe emodin, rhein, curcumin, emodin and chrysophanol were linear in the range of 0.3166.32μg·mL-1（r=1.0000,n=5）,0.53610.72μg·mL-1（r=0.9999,n=5）,0.3256.50μg·mL-1（r=1.0000,n=5）,0.5711.40μg·mL-1（r=1.0000,n=5）,0.4919.82μg·mL-1（r=1.0000,n=5）, respectively. The average recoveries （n=6） were 99.7%, 98.8%, 99.2%, 97.4%, 99.9%, respectively, and RSD was 2.47%, 2.55%, 2.64%, 1.50%, 2.54%. Conclusion： The method is simple and repeatable, which can be applied to determine five ingredients at the same test condition in Jinhuang gel.

关 键 词：金黄凝胶 芦荟大黄素 大黄酸 姜黄素 大黄素 大黄酚 HPLC 

分 类 号：R284.1[医药卫生—中药学]