高效厌氧氨氧化颗粒污泥脱氮特征及EPS分层特性  

Nitrogen Removal Performance and EPS Characteristics of Anammox Granular Sludge with High Activity

作　　者：王衫允[1] 贾方旭[2,3] 靳鹏飞[2,3] 高梦佳 马斌[2,3] 彭永臻[1,2,3] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江哈尔滨150090 [2]北京工业大学北京市水质科学与水环境恢复工程重点实验室,北京100124 [3]北京工业大学北京市污水脱氮除磷处理与过程控制工程技术研究中心,北京100124

出　　处：《中国给水排水》2016年第11期35-39,共5页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51478013);哈尔滨工业大学城市水资源与水环境国家重点实验室开放基金资助项目(QAK201502)

摘　　要：考察了处理低氨氮废水的厌氧氨氧化颗粒污泥的脱氮特征、分层EPS组分及三维荧光光谱特性。结果表明,厌氧氨氧化颗粒污泥表现出高效的厌氧氨氧化活性,对NH+4-N和NO-2-N的平均降解速率分别为0.14和0.15 g/(g VSS·d),去除率分别为80.1%和97.0%;对TN的去除率为65.8%;该过程伴随N_2O的产生和短时积累,峰值浓度为0.92 mg/L,仅占TN转化率的2.52%;另外,p H值能指示厌氧氨氧化反应的终点。对分层EPS组分的分析可知,蛋白质为所有EPS层的主要成分,达226.9 mg/g VSS,占EPS总量的58.9%;绝大部分EPS为TB-EPS,占EPS总量的77.1%。三维荧光光谱显示蛋白质主要为酪氨酸和色氨酸,两者在TB-EPS中的荧光强度分别为165.5和46.1 AU/(mg C·L)。该研究结果为今后厌氧氨氧化污泥颗粒化的研究提供了理论依据。The nitrogen removal performance, EPS characteristics and three-dimensional fluores- cence spectrum of Anammox granular sludge for the treatment of wastewater with low ammonia concentra- tion were investigated. The results showed that Anammox granular sludge exhibited high activity, and the average degradation rates of NH4＋ -N and NO2- -N were 0.14 g/（ gVSS ·d） and 0.15 g/（ gVSS · d） with removal rates of 80.1% and 97.0%, respectively. The removal rate of TN was 65.8%. In this process, N20 was produced with short-time accumulation, and the peak concentration was 0.92 mg/L, accounting for 2.52% of TN conversion rate. In addition, pH value could indicate the end of Anammox reaction. The analysis of different layers of EPS components showed that protein （226.9 mg/gVSS） was the main component in all EPS layers, accounting for 58.9% of the total EPS. TB-EPS accounted for 77.1% of the total EPS. Three-dimensional fluorescence spectrum analysis showed that the protein in EPS was mainly tyrosine and tryptophan, with the fluorescence intensity of 165.5 AU/（ mgC · L） and 46.1 AU/（ mgC ·L）, respectively. These results can provide theoretical basis for the future study on Anammox granular sludge.

关 键 词：厌氧氨氧化 颗粒污泥 PCR—clone 分层胞外聚合物 三维荧光光谱 

分 类 号：X703[环境科学与工程—环境工程]