皮炎洗剂对小鼠慢性变应性接触性皮炎的影响及其作用机制  

Study of the Effect and Mechanism of Dermatitis Lotion in the Treatment of Allergic Contact Dermatitis in Mice

作　　者：刘青[1] 王琼[2] 陈慧[3] 姜誉弘 王丽灵[3] 王思农[1] 

机构地区：[1]甘肃中医药大学中医临床学院,甘肃兰州730000 [2]南京中医药大学附属医院,江苏南京210029 [3]南京中医药大学药学院,江苏南京210023

出　　处：《南京中医药大学学报》2016年第3期279-282,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：甘肃省教育厅高等学校科研项目(2014A-081)

摘　　要：目的评价皮炎洗剂治疗小鼠慢性变应性接触性皮炎(ACD)的疗效。方法采用2,4-二硝基氯苯(DNCB)诱导小鼠背部ACD。60只小鼠随机分为空白对照组、ACD组、阳性组、皮炎洗剂大剂量组(0.4g/mL)、中剂量组(0.2g/mL)、小剂量组(0.1g/mL),每组10只,于致敏后给药,观察各组小鼠第4次激发24h后皮损炎症程度、组织病理切片、炎症细胞数量改变及血清中IFN-和IL-4水平的变化。结果皮炎洗剂中、大剂量能有效减轻ACD小鼠引起的免疫器官肿大、皮损炎症程度,减少皮损组织炎症细胞数量,调节ACD组小鼠血清中-干扰素(IFN-)和白细胞介素-4(IL-4)水平。结论皮炎洗剂对小鼠ACD具有明显的治疗作用,其作用机制可能与皮炎洗剂调节Th1、Th2型细胞因子有关。OBJECTIVE To study the efficacy of dermatitis lotion in the treatment of allergic contact dermatitis in mice.METHODS The 2,4-dinitro chlorobenzene（DNCB）was applied on the dorsum of mice to induce the allergic contact dermatitis models.60 mice were randomly divided into control,model（ACD）,momestasone furoate,dermatitis lotion（0.4,0.2,0.1g/mL）groups,with 10 mice in each group.Mice were sensitized by DNCB and then treated topically with different drugs on lesions.The inflammation degree of skin lesion were observed,histopathological analysis was measured by HE strain and the levels of serum IFN- and IL-4were detected by ELISA.RESULTS The dermatitis lotion of 0.4g/mL restrained the increase of thymus index,alleviated the inflammation degree of lesion on dorsum of mice and reduced the pathological score significantly,compared with the model group.In addition,the dermatitis lotion of 0.4g/mL adjusted the expression of serum IFN- and IL-4to return to normal levels.CONCLUSION These results suggest that dermatitis lotion can be used for the treatment of allergic contact dermatitis（ACD）mainly through the regulation Th1/Th2 cytokines.

关 键 词：皮炎洗剂 小鼠 慢性变应性接触性皮炎 IFN-Γ IL-4 

分 类 号：R285.5[医药卫生—中药学]