基于高盐诱导的SHR大鼠肾损伤探讨中医理论“盐胜血”的机理研究  

Explore the Mechanism of Chinese Medicine Theory"Salt-hurt-blood"Based on High Salt-induced Kidney Injury in SHR Mice

作　　者：李婕[1] 严士海[2] 王欣彤[2] 张思奇[2] 方祝元[2] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210023 [2]南京中医药大学附属医院,江苏南京210029

出　　处：《南京中医药大学学报》2016年第3期287-290,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：教育部博士点基金(20123237110005);国家自然科学基金(81273713;81302902)

摘　　要：目的研究关于高盐诱导的SHR大鼠肾损伤的相关基础,并探讨中医理论"盐胜血"的内涵。方法 WKY大鼠为正常组,SHR大鼠随机等分为2组,分为普通饲料组(SHR)与高盐饲料组(SHR+HS),SHR+HS组大鼠从10周龄开始给予4%高盐饮食。每2周检测尿m-ALB与NAG。20周龄大鼠麻醉后腹主动脉取血,检测血液流变学指标与凝血4项;处死大鼠,取肾脏,分为2部分,一部分中性福尔马林固定,HE病理检测;一部分采用Western blot法检测细胞间粘附因子1(ICAM-1)、血管细胞粘附因子1(VCAM-1)、白介素6(IL-6)的表达情况。结果喂养高盐饲料6周后,尿m-ALB与NAG显著增高(P<0.05)。20周龄时SHR+HS组的全血黏度值与血浆黏度值显著大于SHR组(P<0.05);APTT、PT显著缩短(P<0.05)及FIB显著增加(P<0.05);肾脏病理检测可见SHR+HS组的病变积分值更高,且肾脏炎症因子ICAM-1、VCAM-1、IL-6表达增加(P<0.05)。结论高盐可以增加SHR组大鼠的全血黏度、缩短APTT、PT与增加FIB,增加肾脏炎症因子ICAM-1、VCAM-1、IL-6表达,加重SHR组大鼠的肾损伤,这可能是"盐胜血"与"咸走肾"的部分科学内涵。OBJECTIVE To study the related basic research of high salt-induced kidney injury in SHR rats,and discuss the salt-hurt-blood connotation of traditional Chinese medicine.METHODS WKY rats were set as the normal group.SHR were randomly divided into 2groups：normal group（SHR）and high-salt feed group（SHR＋HS）.SHR＋HS group rats were given4% high salt diet from the 10 th week.Urine trace albumin and NAG in urine were detected every two weeks.On the 20 th week,the hemorheology indexes and coagulation four indices were measured.The rat kidney was divided into two parts.One was fixed with formalin and detected with HE;the other part was used to detect ICAM-1,VCAM-1and IL-6expressions.RESULTS After feeding high-salt feed for 6weeks,urine trace albumin and NAG increased significantly（P〈0.05）.On the 20 th week,the whole blood viscosity and blood plasma viscosity in SHR＋HS group were significantly（P〈0.05）greater than SHR group.APTT and PT were significantly reduced and FIB significantly increased（P〈0.05）.Renal pathology index in SHR＋HS group was higher than SHR group,and the kidney inflammation factors ICAM 1,VCAM 1and IL-6increased（P〈0.05）.CONCLUSION High salt can increase the whole blood viscosity,disorder APTT,PT and FIB,increase the expression of ICAM 1,VCAM 1and IL-6,and strengthen the kidney injury of SHR rats,which might partly explain the scientific connotation of ＂salt-hurt-blood＂ and ＂salty flavor entering the kidney＂.

关 键 词：盐胜血 咸走肾 SHR大鼠 

分 类 号：R285.5[医药卫生—中药学]